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Construction method for presenile dementia white rat animal model

A kind of Alzheimer's disease, animal model technology, applied in teaching models, educational appliances, instruments and other directions, can solve the problems of the application limitation of transgenic animals, unable to truly reflect AD pathological changes and other problems

Inactive Publication Date: 2004-12-29
华中科技大学同济医学院
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Problems solved by technology

Transgenic animal models are the main research progress in recent years, but familial AD is usually caused by polygenic abnormalities, and most of the current transgenic models are transplanted with one or at most two genes or gene fragments, which cannot truly reflect the severity of AD. Pathological changes also need to be improved in behavioral experiments, and epidemiological survey data show that more than 80% of AD patients do not involve gene mutations (BraakH, 1997)
Therefore, the application of transgenic animals is limited

Method used

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  • Construction method for presenile dementia white rat animal model
  • Construction method for presenile dementia white rat animal model

Examples

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example

[0040] Example Illuminated Rats to Construct AD-like Rat Animal Model

[0041] (1) Experimental animals and groups

[0042] Male Wistar rats (provided by the Experimental Animal Center of Tongji Medical College, Huazhong University of Science and Technology), SPF grade, weighing 50-70 grams, 30 rats. Divided into two groups, 15 rats in each group: ① normal group, reared in a conventional environment (12h: 12h), that is, 7:00-19:00 natural light, 19:00-7:00 no light; ② model group, continuous light Method (24h:0h): 7:00-19:00 natural light, 19:00-7:00 light; light intensity: 150 Lux; time: 6 weeks, during which animals can eat and drink freely, and the room temperature is 20-25°C.

[0043] (2) Main reagents

[0044] Acrylamide (Arc), N,N-Methylenebisacryloyl (bis), Tetramethylethylenediamine (TEMED), Sodium Dodecyl Sulfate (SDS), Glycine (Glycine), Bovine Serum Albumin (BSA), tris(Tris), sodium vanitate (Na3VO4), β-phosphoglycerol (β-PG), sodium fluoride (NaF), phosphorylase...

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Abstract

The present invention belongs to the field of medical evaluation and detection technology, and is especially the construction process of Alzheimer's disease (AD) rat animal model. Rat is set in lighting environment of 10-1000 Lux light strength continuously for 1-10 weeks. The model may be used in the research of AD pathogenesis in the early stage or the disease process including beta-amyloid protein increase and congregation, skelemin Tau AD like abnormality and over phosphorylation, etc., the screening of medicine for treating AD, the evaluation of AD treating method and the research of AD pathogenesis.

Description

technical field [0001] The invention belongs to the technical field of medical evaluation and detection, and more specifically relates to a method for constructing an animal model of Alzheimer's disease rats. Background technique [0002] AD is a common neurodegenerative disease, the main symptoms of which are memory loss, low cognitive ability and slow thinking, and progressive aggravation, and finally unable to take care of themselves and die, the course of the disease is generally 5 to 20 years. With the acceleration of the aging process of society, the incidence and number of AD have risen sharply, and it has become a serious disease that threatens the physical and mental health of the elderly, especially the elderly population, and has brought serious social, economic and family problems. [0003] The main pathological changes in the brain of AD patients are a large number of NFTs, a large number of senile plaques, and loss of synapses and neurons. The two corresponding...

Claims

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Application Information

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IPC IPC(8): G09B23/28
Inventor 凌智群王建枝
Owner 华中科技大学同济医学院
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