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Method of producing lactic bacteria and killed lactic bacterial cells belonging to the enterococcus

A technology of enterococcus and manufacturing method, applied in the direction of medical preparations, bacteria, and pharmaceutical formulas containing active ingredients, which can solve problems such as inability to obtain, reduce, and lose effects

Inactive Publication Date: 2004-12-01
河合 康雄
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, even Enterococcus lactic acid bacteria (lactococcus) having the activity of significantly lowering the cholesterol level and triglyceride value can be judged to reduce the activity of the lactic acid bacteria by the existing culture method, thereby failing to obtain the desired lactic acid bacteria. Effect
In addition, the dead cells of Enterococcus lactic acid bacteria, like the above-mentioned live cells, can also significantly reduce the cholesterol level and triglyceride value, but it can be judged that the dead cells obtained by the existing processing method from the above-mentioned live cells , will lose the desired effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0018] 2. Preparation conditions of live bacteria

[0019] The culture conditions of the above-mentioned living bacteria are as follows:

[0020] (i) Hydrogen ion concentration index (pH) of medium components: the pH is in the range of 5.5-7.5.

[0021] (ii) Stirring method: Stir slowly at an average of 5-40 times per minute.

[0022] (iii) Incubation time: in the range of 6-24 hours.

[0023] (iv) Medium composition: containing 1-4% glucose, 0.3-6% yeast extract, 0.1-5% peptone, and 1-5% dipotassium hydrogen phosphate.

[0024] (v) Temperature: under a temperature environment of 30-45°C.

[0025] (vi) Aerobic culture, but oxygen does not need to be specially added.

[0026] 3. Preparation conditions of dead bacteria

[0027] After the living bacterial culture obtained by cultivating under the conditions described in the above 2 is further washed with physiological saline for 1 to 2 times, the bacterial liquid obtained by suspending with physiological saline is subjected ...

experiment example 1

[0035] Live cells of various microorganisms of the genus Enterococcus shown in 1 above, and cells obtained after heat and pressure treatment were administered to mice (18-week-old males, average body weight: 20 g: 10 mice per group) , administered orally continuously within 4 weeks, the dosage is: live bacteria 10 10 pc / day, dead bacteria 10 10 per day, the cholesterol reduction rate in the blood was measured, and the experimental results are shown in Table 1.

[0036] 【Table 1】

[0037] Microorganisms

experiment example 2

[0039] In the same manner as above-mentioned Experimental Example 1, live thallines of various microorganisms of the Enterococcus genus, and the thallines obtained after heating and pressurizing were administered to common rats (male 18 weeks old, average body weight 250g: Every group 7), in the time of 4 weeks oral continuous application, dosage is: live thalline 10 10 pc / day, dead bacteria 10 10 per day, the cholesterol reduction rate in the blood was measured, and the experimental results are shown in Table 2.

[0040] Types of microorganisms

Viable bacteria (%)

Dead bacteria (%)

normal rat

normal rat normal rat normal rat

( * 1) ( * 2)

Enterococcus faecalis K-23

13.0

28.1

38.1

29.3

Enterococcus faecalis K-10

11.0

20.0

22.8

18.2

Enterococcus faecalis K-57

11.4

19.6

21.4

19.0

...

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PUM

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Abstract

A method of culturing is provided which keeps physiology activity of bacterial cell belonging to the Enterococcus genus and also a method of producing killed bacterial cells belonging to the Enterococcus genus. <??>In the method of culturing bacterial cells belonging to the Enterococcus genus, the method cultures bacterial cells belonging to the Enterococcus genus in an aerobic manner under the culturing conditions of (i) pH: 5.5 to 7.5, (ii) stirring method: 5 to 40 times per minute, (iii) temperature conditions: 30 to 40 DEG C, and (iv) culturing time: 6 to 24 hours, and extracts viable bacteria from culture solution.

Description

【Technical field】 [0001] The present invention relates to a method for cultivating lactic acid bacteria of the genus Enterococcus and a method for producing dead cells of lactic acid bacteria of the genus Enterococcus. 【Background technique】 [0002] In recent years, with the increase in the intake of animal fats by Japanese individuals and the evolution of Westernized diet and lifestyle, patients suffering from lifestyle-related diseases such as hyperlipidemia and related arteriosclerosis and adult diseases are increasing. Increase. [0003] For this reason, various drugs are currently available for the treatment of hyperlipidemia. However, side effects such as stomach upset, vomiting, abnormal liver function, acute renal failure, and weakness of limbs have been reported after taking any of the drugs used to suppress this disease, and there are reports of side effects such as stomach discomfort, vomiting, and weakness of limbs when taking other drugs. There are also situa...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61K35/744C12N1/06C12N1/20
CPCC12N1/20C12N1/066A61K35/744
Inventor 河合康雄
Owner 河合 康雄
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