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Water soluble extract of red sage root and its preparation method and use

An extract and the technology of salvia miltiorrhiza, applied in the field of water-soluble extract of salvia miltiorrhiza and its preparation and application, to achieve the effect of good curative effect, sufficient drug source, and less toxic and side effects

Inactive Publication Date: 2012-08-01
CHENGDU DIAO PHARMA GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research report on the effect of salvianolic acid extract on the activity of angiotensin-converting enzyme.

Method used

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  • Water soluble extract of red sage root and its preparation method and use
  • Water soluble extract of red sage root and its preparation method and use
  • Water soluble extract of red sage root and its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 2 Salvianolic acid extract inhibits angiotensin-converting enzyme activity test

[0027] ① Preparation of angiotensin-converting enzyme

Embodiment 2

[0028] Take rat lung tissue, wash with 10mmol / 1 ice potassium phosphate buffer, cut into small pieces, and beat into a homogenate with a homogenizer. The homogenate was centrifuged at 5000g for 10 minutes at 4°C, and the precipitate was discarded. The supernatant was dialyzed overnight with the above ice potassium phosphate buffer solution at low temperature, 2 liters each time, and the solution was changed 4 times. Then centrifuge at 40,000g for 40 minutes at 4°C, discard the precipitate, and the supernatant is the enzyme extract, which is aliquoted and stored at -20°C for future use.

[0029] ② Salvianolic acid extract T 1 Determination of the effect on angiotensin converting enzyme activity

[0030] Take 10ug / ml, 50ug / ml, 100ug / ml different concentrations of salvianolic acid extract T 1 10 μl and 10 μl of enzyme extract (about 10 μg of total protein) were reacted at 37°C for 30 minutes. The blank control was 20 μl of buffer, and the negative control was 10 μl of buffer a...

Embodiment 3

[0037] Experimental rats were divided into 4 groups: salvianolic acid extract T 1 group (hereinafter referred to as Danshen T 1 group, n=6), systolic blood pressure > 18kPa, daily gavage of Danshen extract T 1 (500mg / kg) for 4 consecutive weeks; positive group (n=4), systolic blood pressure > 18kPa, daily intragastric administration of ramipril (1mg / kg) for 4 consecutive weeks; sham operation group (n=4), systolic blood pressure 18kPa, administered with the same volume of normal saline as the drug, for 4 consecutive weeks, respectively, after administration 2 The blood pressure of the rats was measured at 1 week and 4 weeks after administration. The results are shown in Table 1.

[0038] Table 1 Salvianolic acid extract T 1 Effects on Blood Pressure of Renal Hypertensive Rats

[0039]

[0040] Note: #P* P** P<0.01, compared with before taking medicine

[0041] It can be seen from the results in Table 1 that there was a significant difference in blood pressure between...

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Abstract

A water-soluble extract of red sage root containing danshinolic acid B, danshensu and proshikonic acid, its preparing process, and its application in preparing the angiotonin converzyme depressant medicines for treating hypertension, and hypertension accompanied by heart failure or diabetes or nephrosis are disclosed.

Description

technical field [0001] The present invention relates to an extract of medicinal raw material and its preparation method and application. Specifically, it is a water-soluble phenolic acid extract obtained from the medicinal raw material Salvia miltiorrhiza, the extraction and preparation method of the extract, and an angiotensin-converting enzyme inhibitor drug using the extract as an effective medicinal ingredient, which can be used as a therapeutic Drugs for high blood pressure, heart failure, diabetic kidney disease and other diseases. Background technique [0002] Salvia miltiorrhiza is the dried rhizome of Salvia miltiorrhiza, a plant of the Labiatae family, and is a commonly used traditional Chinese medicine in clinical practice. The chemical composition of Salvia miltiorrhiza is mainly composed of fat-soluble components and water-soluble components. Among them, the fat-soluble components are represented by tanshinone. Pharmacological and clinical studies have confirme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C59/52C07C51/47C07D307/80A61K31/343A61P9/12A61P3/10A61P13/12
Inventor 高小平徐大勇刘忠荣李伯刚
Owner CHENGDU DIAO PHARMA GROUP
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