Determination of one section urea degraded mycoplasmos DNA sequence (5) specificity and its application

A technology of DNA sequence and mycoplasma, which is applied in the field of biomedicine to achieve the effect of low cost and easy operation

Inactive Publication Date: 2004-07-21
KUNMING HUANJI BIOLOGICAL CHIP IND
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Ureaplasma urealyticum is a pathogen that frequently infects the human genitals and can cause many genitourinary diseases. Ac...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Determination of one section urea degraded mycoplasmos DNA sequence (5) specificity and its application
  • Determination of one section urea degraded mycoplasmos DNA sequence (5) specificity and its application
  • Determination of one section urea degraded mycoplasmos DNA sequence (5) specificity and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] Firstly, the nucleotide sequence of urealyticum was searched in the gene database with the keyword of ureaplasma urealyticum. A total of several closely related nucleotide sequences were obtained, and these nucleotide sequences were compared with the nucleotide sequences (nr) in the gene database using the Blast online software on the NCBI network to exclude non-specific nucleotide sequences, A 1 Kb specific nucleotide sequence was obtained. More than 20 pairs of PCR primers were designed based on this sequence, DNA was extracted from clinical specimens, PCR amplification was performed, and a pair of sequences and primers suitable for PCR amplification were screened out. The products obtained by PCR amplification were consistent with the expected size (see Fig. 1. The serial numbers in the figure are: 1. Standard molecular weight DNA, the molecular weights from top to bottom are: 622, 527, 404, 309, 242 / 238, 217, 201, 190, 180, 160+160, 147+147, 123; 2.4.5 Clinical neg...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A determination to the specificity of a urea-decomposing mycoplasma DNA sequence (5) segment includes searching and analyzing gene database to determine the specificity of said DNA sequence (288 bp) segment and its PCR amplification primer, PCR amplificating and cloning to human genetic tract's urea-decomposing mycoplasma infective specimen to obtain the sequence, sequencing it to prove that it is consistant with the predicated sequence, biological information analysis, and gene chip cross analysis to prove the specificity of said sequence to urea-decomposing mycoplasma. It can be used as the probe of gene chip diagnosis and the test sequence of PCR amplification to diagnose human mycoplasma.

Description

Technical field: [0001] The invention relates to specific determination and application of a Ureaplasma urealyticum DNA sequence (5), which belongs to the technical field of biomedicine. Background technique: [0002] Ureaplasma urealyticum is a pathogen that frequently infects the human genitals and can cause many genitourinary tract diseases. Accurate diagnosis is the basis for immediate and reliable treatment. Currently, it can only be diagnosed according to clinical indications. PCR and gene chip diagnosis is a fast and accurate detection method. Diagnostic methods of this type rely on the availability of specific nucleic acid sequences. Invention content: [0003] The invention is to determine a section of ureaplasma-specific nucleotide sequence and its PCR primer through bioinformatics and molecular biology techniques. The sequence and corresponding PCR primers can be used to establish a gene chip and PCR method for diagnosing mycoplasma urealyticum. [0004] The ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07H21/04C12Q1/68
Inventor 谭德勇朱宝生
Owner KUNMING HUANJI BIOLOGICAL CHIP IND
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap