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Pig decoupling protein gene 2nucleotide sequence and detection method of its polymorphism

A technology of nucleotide sequence and detection method, which is applied in the field of detection of nucleotide sequence of porcine uncoupling protein gene 2 and its polymorphism, and can solve the problems that the structure and function need to be further studied.

Inactive Publication Date: 2004-07-21
ZHEJIANG UNIV
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Problems solved by technology

Recently, a specific expression of UCP4 has been found in human brain tissue, and the gene is located at the position of human 6p11.2-q12, but the structure and function need to be further studied

Method used

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  • Pig decoupling protein gene 2nucleotide sequence and detection method of its polymorphism
  • Pig decoupling protein gene 2nucleotide sequence and detection method of its polymorphism
  • Pig decoupling protein gene 2nucleotide sequence and detection method of its polymorphism

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Embodiment Construction

[0018] The isolated DNA molecule includes: a nucleotide sequence encoding porcine UCP2 protein activity, SEQ ID No.3, and the nucleotide sequence is the porcine UCP2 exon 3-4 segment and intron 3 segment sequence ; The nucleotide sequence has at least 89% homology with the nucleotide sequence of the human corresponding sequence from nucleotide 3 to 377; or the nucleotide sequence can Hybridize with the corresponding human sequence from nucleotides 3 to 377. There is a polymorphic site caused by the T / G base substitution at position 272 in the intron 3 segment sequence of the isolated DNA molecule. A detection method for the analysis of the polymorphic site was established

[0019] The steps of the polymorphism detection method are as follows:

[0020] 1) Add 35-40 μl of 10% SDS to 700 μl of anticoagulated blood, shake vigorously at room temperature and extract twice with an equal volume of saturated phenol, add 0.6-0.7 times the volume of isopropanol to the aqueous phase, mi...

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Abstract

A nucleotide sequence of hog uncoupling protein gene 2 and the method for testing its polymorphism are disclosed. Said nucleotide sequence, SEQ ID No.3, can code the hog UCP2 protein, is the sequence in the segment 3-4 of hog UCP2 exon and the segment 3 of intron, and has 89% homology with the nucleotide sequence of human relative sequence. It can be used to research the growth and energy conversion of hog.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a method for detecting the nucleotide sequence of porcine uncoupling protein gene 2 and its polymorphism. Background technique [0002] As early as the 1970s, it was discovered that hibernating mammals (such as bears and cold-resistant rodents) can use brown adipose tissue (BAT, also known as red fat) to generate heat to maintain body temperature. Scientist at the National Center for Scientific Research in Paris, France ICQUIER By comparing the changes in mice in hot and cold environments, a class of proteins with a molecular weight of 32kD and unknown functions were discovered. N ICHOLLS He and his colleagues injected the mixture of labeled cells into BAT and found that there is a pore with strong proton permeability in the mitochondrial inner membrane of BAT, which can dissociate the oxidative phosphorylation coupling of the respiratory chain to gen...

Claims

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Application Information

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IPC IPC(8): C12N15/12C12Q1/68
Inventor 徐宁迎赵兴波郭晓令章胜乔
Owner ZHEJIANG UNIV
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