Growth hormone conjunctive protein activity determination kit, preparation and determination method

A growth hormone and binding protein technology, which is applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of no detection of growth hormone binding protein activity, no introduction, and difficult measurement operation

Inactive Publication Date: 2004-06-09
HUAQIAO UNIVERSITY
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Problems solved by technology

The methods for measuring GHBP activity mentioned in these few papers have guiding and reference significance for general hospitals and research units to measure GHBP activity, but referring to these methods is still difficult for specific implementation of the measurement operation
In short, advanced detection technicians have some understanding of the technology for measuring GHBP activity, but the implementation of the detection still needs to do a lot of experimentation and exploration, and ordinary detection technicians c

Method used

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Embodiment Construction

[0019] The present invention will be further described in detail below in conjunction with Table 1 and Table 2 and the embodiment, wherein Table 1 is a schematic diagram of the steps of the embodiment of the present invention, and Table 2 is a list of the order and quantity of determination samples that must be listed in the kit instruction manual.

[0020] Dilute with common buffer before measuring operation 125 I-hGH, take 100μl while diluting 125 I-hGH is tested on a radioactive counter until the diluted 125 I-hGH counts up to about 20000 cpm per 100 μl.

[0021] 100 μl of 125 I-hGH.

[0022] Set 6 total counting tubes, the purpose is to make the average radioactivity count of the total counting tubes accurate, and to examine the accuracy of the sample addition operation. Add 100μl to each total counting tube 125 In addition to I-hGH, add 400 μl of analysis buffer, and the total volume of the total incubation reaction solution is 500 μl.

[0023] Set 6 total "non-spec...

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Abstract

This invention discloses a determination kit box combining with protein testing the growing hormone activity in and animals and its preparation and test method characterizing that the box is composed of a solution prepared by a labeled growing hormone, unlabeled growing hormone, glucose enveloped carbon, general buffer solution and analytical solution and a specification. The method is that apart from setting 4-8 NSB tubes, two correspondent NSB tubes are specially designed for every sample to make the test more accurate. The glucose enveloped carbon preparation and quatily are necessary conditions for the accurate test.

Description

1. Technical field [0001] The invention relates to a medical index assay kit for assaying growth hormone binding protein activity in human and animal serum and its preparation and assay methods. 2. Background technology [0002] Growth hormone binding protein (GHBP) in human and animal serum plays an important role in the distribution, metabolism and physiological activities of growth hormone. GHBP is a single-chain protein composed of 238-246 amino acids, with a molecular weight of about 61kDa, an acidic protein, and an isoelectric point of about 5.0. The main physiological function of GHBP is to combine with the growth hormone produced by the pituitary gland to transport the growth hormone to the tissue cells of the whole body in the blood, which can effectively store the growth hormone and prolong the activity of the growth hormone. GHBP can also combine with the growth hormone and the growth hormone receptor The increase and decrease of growth hormone regulate the physi...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/534G01N33/60G01N33/74
Inventor 贺淹才
Owner HUAQIAO UNIVERSITY
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