Growth hormone conjunctive protein activity determination kit, preparation and determination method

Abstract

This invention discloses a determination kit box combining with protein testing the growing hormone activity in and animals and its preparation and test method characterizing that the box is composed of a solution prepared by a labeled growing hormone, unlabeled growing hormone, glucose enveloped carbon, general buffer solution and analytical solution and a specification. The method is that apart from setting 4-8 NSB tubes, two correspondent NSB tubes are specially designed for every sample to make the test more accurate. The glucose enveloped carbon preparation and quatily are necessary conditions for the accurate test.

Description

1. Technical field [0001] The invention relates to a medical index assay kit for assaying growth hormone binding protein activity in human and animal serum and its preparation and assay methods. 2. Background technology [0002] Growth hormone binding protein (GHBP) in human and animal serum plays an important role in the distribution, metabolism and physiological activities of growth hormone. GHBP is a single-chain protein composed of 238-246 amino acids, with a molecular weight of about 61kDa, an acidic protein, and an isoelectric point of about 5.0. The main physiological function of GHBP is to combine with the growth hormone produced by the pituitary gland to transport the growth hormone to the tissue cells of the whole body in the blood, which can effectively store the growth hormone and prolong the activity of the growth hormone. GHBP can also combine with the growth hormone and the growth hormone receptor The increase and decrease of growth hormone regulate the physi...

AI Technical Summary

Problems solved by technology

The methods for measuring GHBP activity mentioned in these few papers have guiding and reference significance for general hospitals and research units to measure GHBP activity, but referring to these methods is still difficult for specific implementation of the measurement operation

In short, advanced detection technicians have some understanding of the technology for measuring GHBP activity, but the implementation of the detection still needs to do a lot of experimentation and exploration, and ordinary detection technicians cannot use or directly detect according to the technical content of the retrieved literature

In addition, a few other literatures mentioned the content of measuring human GHBP, but did not introduce the specific method. Generally, foreign reagents or foreign kits for measuring GHBP were used.

After searching, some biotechnology companies in China have kits for the determination of growth hormone binding protein, all of which are foreign products, and there is no kit for determining the activity of growth hormone binding protein.