Plant extract for preventing and treating diabetes, and preparing method and pharmaceutic use thereof
A technology of plant extracts and diabetes, applied in the direction of drug combinations, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve problems that have not been reported
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Embodiment 1
[0020] Embodiment 1: Preparation 1 of plant extract
[0021] Taxus coarse powder 60kg, reflux extraction with 4 times of volume 90% ethanol three times, the merging extract is concentrated into thick solution; 2 O two-phase extraction, combined the water layer, filtered, put on a macroporous resin column (D101 type, domestic), and eluted with distilled water and hydrous ethanol (ethanol: 5%-20%) gradient, and each eluent was tested with a differential test The eluents that showed a positive reaction were combined and concentrated to dryness in a rotary evaporator, recrystallized from ethanol, filtered to obtain a crystalline powder, dried, and dried at 70°C to obtain a plant extract (light yellow powder ), yield 0.10%. The HPLC method is used for detection, and the content of myo-inositol derivatives reaches 81%.
Embodiment 2
[0022] Embodiment 2: Preparation 2 of plant extract
[0023] Taxus coarse powder 70kg, extract three times at 50 ℃ with 3 times volume of 80% methanol, combine extracts, concentrate into slurry solution; Ethyl ester / H 2 O two-phase extraction, combined water layer, filtered, put on macroporous resin column (MN-200 type), gradient elution with distilled water and hydrous ethanol (ethanol: 5%-20%), and each eluent was used for identification test The eluents that showed a positive reaction were combined, concentrated to dryness under reduced pressure in a rotary evaporator, recrystallized from acetone, filtered to obtain a crystalline powder, dried, and dried at 70°C to obtain a plant extract (off-white powder ). Yield 0.12%. The HPLC method is used for detection, and the content of myo-inositol derivatives reaches 75%.
Embodiment 3
[0024] Embodiment 3: Preparation 3 of plant extract
[0025] Taxus coarse powder 65kg, extracted three times with 4 times the volume of 75% acetone at 45 ° C, the combined extracts were concentrated into a thick solution; concentrated to dryness under reduced pressure with a rotary evaporator, and dichloromethane / H 2 O Two-phase extraction, combined water layer, filtered, put on activated carbon (pharmaceutical specification) column, gradient elution with distilled water and hydrous ethanol (ethanol: 5%-30%), each eluent was identified with differential test solution , combined the eluents showing a positive reaction, concentrated to dryness under reduced pressure in a rotary evaporator, recrystallized from methanol, filtered to obtain a crystalline powder, dried, and dried at 70°C to obtain a plant extract (light yellow powder) , yield 0.13%. The HPLC method is used for detection, and the content of myo-inositol derivatives reaches 66%.
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