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Method for cultivating nostoc commune

A technology of ground fungus and culture solution, applied in the biological field, can solve the problems of limited resources, limited food demand of ground fungus, artificial breeding of ground fungus, etc., and achieve the effect of enriching material life, convenient operation and high yield

Inactive Publication Date: 2003-10-22
INST OF AQUATIC LIFE ACAD SINICA
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AI Technical Summary

Problems solved by technology

[0003] However, due to the very limited resources of wild fungus, it greatly limits people's food demand for fungus, and there has been no report on the artificial cultivation of fungus

Method used

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Embodiment Construction

[0037] Its steps of the method for cultivating ground fungus are as follows:

[0038] 1. Culture medium preparation: each liter contains 20-60mg of dipotassium hydrogen phosphate, 40-80mg of magnesium sulfate, 20-40mg of calcium chloride, 4-8mg of citric acid, 5-9mg of ferric ammonium citrate, and ethylenediaminetetraacetic acid Sodium salt 1-2mg, sodium carbonate 5-30mg, trace elements 0.5-3ml, the rest is water; sodium nitrate 200-1500mg / L can also be added to the above culture medium.

[0039] Among them, trace elements are contained per 1000 ml: boric acid 1.5-3g, manganese chloride 1-2g, zinc sulfate 0.1-0.25g, sodium nickelate 0.2-0.4g, copper sulfate 0.02-0.1g, cobalt nitrate 0.03-0.05g, The rest is water.

[0040] The prepared culture solution should preferably be sterilized at a high temperature of 118-122°C for 20 minutes, and then used after cooling, or can be sterilized by filtration.

[0041] 2. Preparation of algae species: Wash the fungus with 70%-95% ethanol ...

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PUM

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Abstract

The nostoc commune culture method includes the following steps: soaking nostoc commune in ethyl alcohol solution, washing in sterilized culture liquor, placing the above-mentioned material into a homogenizer, grinding to obtain a paste-like material, then inoculating it into culture liquor, its temp. is controlled at 10-40 deg.C, and the light intensity is controlled at 10-1800 micro E.m(-2).s(-1), changing culture liquor once every 10-20 days until the spherical body of noctoc commune is grown to the required diameter. Said invention is convenient for operation, and its yield is high.

Description

Technical field: [0001] The present invention relates to biotechnology, and more particularly to the cultivation of microalgal organisms. Background technique: [0002] Nostoc is a kind of algae group with wide distribution, complex ecological environment and various morphological structures in the cyanophyta Cyanophyta Cyanophyceae Cyanophyceae segmental algae order Hormogonales Nostocaceae. , leaf-like, filamentous, bubble-like and other shapes, hollow or solid, floating or growing. The protophyte consists of many fine algal filaments with a diameter of a few microns assembled into a group, and the shape of the group varies depending on the "species", such as Nostoc flageliforme Kuetz, Nostoc commune, Nostoc sphaeroides In the fresh state, they are hair-like, flat ear-shaped or small ball (bead)-shaped, and they shrink to varying degrees after drying, all of which have rich nutritional value. It has been recorded in medical books such as "Shaanxi Chinese Herbal Medicine"...

Claims

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Application Information

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IPC IPC(8): C12N1/12
Inventor 邓中洋胡征宇刘国祥阎春兰李运广韩丹翔毕永红赵先富徐敏
Owner INST OF AQUATIC LIFE ACAD SINICA
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