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Tissue culture reproduction method of common nepenthes

A tissue culture and Nepenthes technology, applied in the field of culture medium, can solve problems such as no reports of patent applications, and achieve strong resistance, high stability, and good growth effects

Inactive Publication Date: 2003-09-17
SOUTH CHINA PLANT INST CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are no ornamental varieties or hybrids of Nepenthes widely introduced at home and abroad for tissue culture and large-scale production, and there are no reports of patent applications

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1. Materials: The terminal buds or segmented stem segments of hybrid Nepenthes (Nepenthes x Dyeriana) are used as explants.

[0016] 2. The method of explant induction material disinfection: in the growing season, select the 3-4 cm long terminal buds or stem segments with lateral buds of vigorously growing Nepenthes as explants, rinse them under tap water, and then put them on the Soak in 70% alcohol for 30 seconds, then disinfect with 0.1% mercuric acid solution for 15 minutes, rinse with sterile water 4 to 5 times, cut off the brown part of the wound at the base, and inoculate it into the medium 1 / 2MS+6-benzylpurine 1.0 mg / L + NAA 0.05 mg / L + 0.05% activated carbon. After 5 days, transfer to a new medium of the same kind to prevent browning, and adventitious buds can be formed in about 40 days.

[0017] 3. Induction and subculture of adventitious buds: the induced adventitious buds were cut and then subcultured in the same medium for proliferation. Generally, 40 day...

Embodiment 2

[0021] The terminal buds or segmented stems of Nepenthes misabilis were used as explants. The basic operation method is consistent with embodiment 1. But its adventitious bud induction and cluster bud proliferation medium is 1 / 2MS + 6-benzyl purine 2.0 mg / L + naphthalene acetic acid 0.1 mg / L + 0.05% activated carbon, and the multiplication rate is 2-3. The survival rate of transplanted test tube seedlings under the same conditions was 85%.

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PUM

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Abstract

A tissue culture method for reproducing nepenthes which has unique shape for appreciation, its several reproduction methods, its culture media, and the method for disinfecting its explant are disclosed. Its advantage is high speed and effect.

Description

technical field [0001] The invention relates to a tissue culture propagation method of nepenthes and a used culture medium. technical background [0002] Nepenthes (Nepenthes L.) is also known as piglet cage, caterpillar plant, water bucket, doll bottle, fortune and treasure, jug plant, etc. In our country, people have given it an auspicious business name "Bag Ping An ". It belongs to the genus Nepenthes of the Nepenthes family, the only genus of undergraduate plants, with more than 70 species, distributed in tropical regions of the Eastern Hemisphere, such as Southeast Asia, Australia, Africa, Madagascar and other places, introduced to the UK in 1789, and then mainly in Europe It is widely cultivated in botanical gardens for ornamental purposes. The first horticultural cultivar of Nepenthes was bred in 1882: N. coccinea. By the middle of the 20th century, the breeding, reproduction and production of Nepenthes began to be industrialized, and a large number of ornamental v...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 段俊曾宋君
Owner SOUTH CHINA PLANT INST CHINESE ACAD OF SCI
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