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Anti-mastitis causative-agent composite yolk antiboy for milk cow and its preparation and use

A technology for yolk antibody and mastitis, applied in the direction of antibodies, antibacterial drugs, diseases, etc., to achieve the effect of improving quality and easy industrialization

Inactive Publication Date: 2003-08-27
重庆和润实业(集团)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the prior art, there is no report of using dairy cow mastitis pathogenic bacteria (staphylococcus, streptococcus, G-bacillus) as an antigen to immunize healthy hens, collecting eggs produced by them, and then extracting anti-MasIgY from the eggs

Method used

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  • Anti-mastitis causative-agent composite yolk antiboy for milk cow and its preparation and use
  • Anti-mastitis causative-agent composite yolk antiboy for milk cow and its preparation and use

Examples

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Effect test

Embodiment 1

[0032] Example 1: Dairy cow mastitis pathogens (Staphylococcus, Streptococcus, G-bacillus) compound egg yolk antibody products with antibody activity can be obtained by the following steps: Preparation of dairy cow mastitis pathogens (Staphylococcus, Streptococcus, G-bacillus) ) Antigen, immunize healthy hens with the antigen, then collect the eggs laid, and then extract the anti-MasIgY from the eggs laid.

Embodiment 2

[0033] Example 2: Preparation of bacterial antigens, pathogenic bacteria strains, expanded culture in nutrient broth medium for 24 hours. Collect the bacteria, add complete Freund's adjuvant at a weight ratio of 1:1, and emulsify the bacteria antigen.

Embodiment 3

[0034] Example 3: Preparation of Capsular Subunit Antigen: The strain was inoculated in fresh beef broth at a ratio of 1:100, cultured at 37°C for 24 hours, then inactivated in a 60°C water bath for 1 hour, shaken several times and placed in the refrigerator overnight, and centrifuged at 4000g the next day 25min, collect the supernatant, add an appropriate amount of CTAB (pre-dissolved in a water bath), shake it in the refrigerator overnight, centrifuge at 4000g for 45min, discard the supernatant, collect the precipitate, grind with 2.5% deionized water of the original bacterial solution, and add the same amount of 2mol / L of CaCl 2 Solution. Shake for 1 hour, add 25% (v / v) pre-cooled absolute ethanol by volume, shake well, overnight in the refrigerator, centrifuge at 4000g for 25 minutes, discard the precipitate, collect the supernatant, and continue to add absolute ethanol to make the total amount of ethanol 80 %(V / V), overnight in the refrigerator, centrifuge at 4000g for 45min...

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Abstract

The invention refers to an anti-cow mastitis pathogenicbacteria compound yolk antibody (anti-MasIgY), the making method and its application of making drugs curing relative diseases caused by infection of cow mastitis or feed additive. Test anti-MasIgY according to SDS-PAGE electrophoresis (7.5% separating glue), its chart showing single band; colorize blue by R250; use immune mark Western-blotting to test the clear peculiar ferment-dyed stripes appearing in medium-high molecular weight banjd; adopts the biochemical method of double-contracted urea to determine protein concentration. The method: making the cow mastitis pathogenicbacteria antigen, make immunity on healthy hens by the antigen, collecting eggs, and then extracting anti-MasIgY from the eggs.

Description

Technical field [0001] The invention relates to an egg yolk antibody substance with antibody activity, an anti-cow mastitis pathogenic bacterium egg composite yellow antibody (anti-MasIgY) product, and relates to its preparation process and application in veterinary medicine and feed additives. technical background [0002] Mastitis (Mastitis) is an inflammation of the mammary glands of dairy cows, which is caused by a variety of specific pathogenic microorganisms. It is one of the main hazards of the dairy industry in the world, a common disease in dairy cows, and the disease that causes the most serious economic losses in the dairy industry. According to foreign reports, the incidence of mastitis in dairy cows is about 25%-60%. About 50% of the 11 million lactating dairy cows in the United States suffer from mastitis. The economic loss caused is estimated to reach US$2 billion per year. In Japan The prevalence of mastitis was 45.1%. The incidence of mastitis in dairy cows in my...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/395A61P15/14A61P31/04C07K16/02
Inventor 雁杰陈君
Owner 重庆和润实业(集团)有限公司
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