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Wheat 1B†ù14 gene, its coded protein and its promotor

A 1bx14, promoter technology, applied in the field of PCR primers, can solve problems such as not found

Inactive Publication Date: 2003-07-09
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The genes of 1Bx14 and 1By15 subunits and their promoters have not been isolated and sequenced in other laboratories at home and abroad, and no patents related to the genes of 1Bx14 and 1By15 subunits and their promoters have been found in the international patent database.

Method used

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Examples

Experimental program
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Embodiment Construction

[0031] ① Cloning and sequencing of the full-length coding region of the 1Bx14 subunit gene. In order to obtain the full-length coding region of the 1Bx14 subunit gene from the genome of the high-quality wheat variety Xiaoyan 54, we designed a pair of PCR primers P3 and P4 (P3, 5'-ATCACCCACAACACCGAGCA -3' (SEQ ID NO: 6); P4, 5'-AGCTGCAGAGAGTTCTATCA-3' (SEQ ID NO: 7)). Using this pair of primers and genomic PCR reaction, we obtained amplified fragments of the full-length coding regions of six high molecular weight glutenin genes in Xiaoyan 54 (the fragment lengths range from 1.9 to 2.5 kb). After cloning the above fragments into the pGEM-T easy (Promega) vector, more than 200 positive clones were obtained. 50 positive clones were selected from them and the insert fragments contained in them were sequenced. It was found that the amino acid sequences obtained after translation of the insert fragments in two clones (pBx14-2, pBx14-42) were consistent with the published ones from ...

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PUM

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Abstract

The present invention provides a gene for coding wheat 1BX14 subunit, protein coded by said gene and promotor for inducing said gene to make expression. Said invented IBX14 subunit gene had best possess SEQ ID NO:1 indicated nucleotide sequence, and the coded protein had best possess SEQ ID No.2 indicated amino acid sequence. Said invention also provides the method for identification of wheat strain containing 1BX14 gene and DNA primer used for said method.

Description

technical field [0001] The invention relates to the wheat 1Bx14 gene, its encoded protein and its promoter; the invention also relates to a method for identifying a wheat variety (line) containing the 1Bx14 gene and PCR primers used in the method. technical background [0002] Common wheat (hereinafter referred to as wheat) is one of the most important food crops in the world, and various products (such as bread, steamed buns, noodles, etc.) prepared from wheat flour are the daily food of the general public. Due to the importance of wheat products, governments and scientists of various countries have always paid great attention to the genetic improvement and genetics research of wheat. Whether in traditional genetics or in emerging functional genomics research, the genetic basis and improvement of wheat grain quality traits have always attracted the attention of researchers from all over the world. Wheat grain quality traits include many aspects, but the most important one ...

Claims

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Application Information

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IPC IPC(8): C07H21/04C07K14/415C12N15/29C12Q1/68
Inventor 王道文李振声李滨万永芳李文凤张学勇董玉笙刘坤凡
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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