Structural method for nitrogen-fixing plants combined symbiont

Abstract

The present invention relates to joint nitrogen-fixing plant symbiosis technology and is the constructing method of nitrogen-fixing plants. The present invention combines the observation and test in pure culture condition and large scale field simulation. The specific operations include the selection of fungus and bacterium variety; and the combination of mycorhiza fungi, nitrogen fixing, bacteria and symbiotic nitrogen-fixing plants to construct symbiont. The overwhelming combination includes VA mycorhiza fungi, exogenous mycorhiza fungi and nitrogen fixing bacteria separated from root of nitrogen-fixing tree. The said method can utilize the superiority of both nitrogen-fixing plant and microbes to restore the ecological environment of fragile zone.

Description

technical field [0001] The invention relates to a nitrogen-fixing plant joint symbiosis technology, in particular to a method for constructing a nitrogen-fixing plant joint symbiosis. Background technique [0002] Nitrogen is the main element of protein and a large amount of nutrients necessary for plant growth. Although the air contains nearly 80% nitrogen, it cannot be directly absorbed and utilized by higher plants. The energy consumption of artificially synthesizing nitrogen fertilizers is huge, and the environmental pollution caused by unreasonable excessive application is also becoming more and more serious. People gradually realize that agriculture and forestry It is not a good strategy to completely rely on chemical fertilizers for industrial production, so the research on biological nitrogen fixation has attracted much attention. The most frequent limiting factor for biological nitrogen fixation in poor soils is phosphorus, which is because phosphorus can only be c...

AI Technical Summary

Problems solved by technology

In the past two decades, the research on the two-in-one symbiotic system of mycorrhizae and nodules has made remarkable progress, especially in the establishment, optimization and mechanism of the symbiotic relationship from the aspects of genetic characteristics, morphological anatomy, and physiological ecology. Research, the technology of promoting the formation and application of symbionts through artificial inoculation has also developed rapidly, and the application potential of mycorrhizae and root nodules has been shown in the practice of agricultural and forestry production; Roots, nitrogen-fixing plants and rhizobia or actinomycetes symbiotically form root nodules, and then when applied on barren land, the nutrient symbiosis environment between plants and microorganisms cannot be formed optimally, and the excellent characteristics of nitrogen-fixing tree species can be exerted to greatly satisfy them. individual growth needs

Many scholars have recognized the joint symbiosis phenomenon of higher plants, root nodules, and mycorrhizas long ago, and the existence of nitrogen-fixing plant mycorrhizae is a concrete manifestation of this joint symbiosis phenomenon; existing studies have shown that mycorrhizal fungi and There is mutual promotion between symbiotic nitrogen-fixing bacteria, especially mycorrhizal bacteria have obvious synergistic effect on the nitrogen-fixing effect of nitrogen-fixing bacteria. The existence of mycorrhizae promotes the growth of host plants and improves the micro-ecological environment of plant rhizosphere; However, this type of research is basically limited to between VA bacteria or exophytic bacteria, and mostly stays in the description of the phenomenon, and is not carried out under pure culture conditions. It is attributed to the synergistic effect of double inoculation. Blind conclusions will inevitably lead to the inability to make the best use of nitrogen-fixing plant resources and mycorrhizal technology, and the effect of giving full play to the symbiosis of nitrogen-fixing plants and microorganisms is not ideal, and the ecological restoration effect in fragile areas is not good.