Reagent box for fast detecting methyl amphetamine and its preparation process

Abstract

The detection test paper strip includes four parts: the filtering sample paper, glass fiber of antibody of amphetamine methyl marked by colloidal gold, in T line area (detection area), coated by amphetamine methyl-BSA and in C line area (quality control area), film of pyroxylic coated by ovine antibody for anti the antibody of rat, absorbent paper. The formula of the process solution of the filtering sample paper is as follows. Brij 0.05-3 wt%, Triton X-100 0.01-2%, BSA 0.5-3%, NaCI 3-9%, pure water 90-97%, PB buffer solution 0.01-0.03 mmol. The invention provides the features of high sensitivity, strong specificity, good repeatability, fast and accurate.

Description

1. Technical field: [0001] The invention relates to a detection kit made by using anti-methamphetamine monoclonal antibody and colloidal gold immunochromatography technology, and a method for detecting methamphetamine in human urine by using the kit. It belongs to the field of medical testing supplies. 2. Background technology: [0002] Methamphetamine (Methamphetamine, also known as methamphetamine, methamphetamine, commonly known as "ice") is a therapeutic drug that mimics sympathetic stimulation. It belongs to the central nervous system stimulant and is also a psychotropic drug strictly controlled by the world and my country. It is an α and β receptor stimulant. Acute administration of higher concentrations will lead to increased excitement of the central nervous system, resulting in abnormal euphoria, increased sensitivity, faster heartbeat and breathing, and perceived energy. Long-term or heavy use will produce tolerance and physical dependence, and lead to abuse. Fu...

AI Technical Summary

Problems solved by technology

Chromatographic and immunological methods are costly, inconvenient, and time-consuming to detect drugs, especially for front-line public security personnel in drug inspection and anti-drug and monitoring work of anti-drug rehabilitation institutions in small cities.

There are relevant similar detection method bibliographical reports both at home and abroad (U.S. Patent 5,026,827. Use the conjugate of N-(4-aminobutyl) amphetamine and protein to produce the antibody that can combine with methamphetamine; Kur