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Method for assortative breeding cotton variety by molecular-marking, auxiliary modifying and backcross aggregate

A molecular marker-assisted, cotton variety technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, and microbial assay/inspection, etc., can solve problems such as accelerating the breeding process, and achieve the effect of improving fiber quality

Inactive Publication Date: 2003-03-26
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

So far, there is no report on the use of this type of molecular markers in assisted breeding selection to accelerate the breeding process

Method used

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  • Method for assortative breeding cotton variety by molecular-marking, auxiliary modifying and backcross aggregate
  • Method for assortative breeding cotton variety by molecular-marking, auxiliary modifying and backcross aggregate

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Embodiment Construction

[0016] Taking Simian No. 3, a variety popularized in the Yangtze River Basin, as the recurrent parent, and Shanxi 94-24 and 7235 lines as the target trait donors (non-recurrent parents), the modified backcross aggregation breeding method of molecular marker-assisted selection was used to screen for high-yield breeding. , insect-resistant, high-quality new strains.

[0017] Simian No. 3 is an upland cotton variety widely planted in the Yangtze River Basin. It has high yield, high resistance to Fusarium wilt, good adaptability, and ideal plant type, but the fiber quality is average and it is not resistant to insects. Shanxi 94-24 is the result of the Cotton Research Institute of Shanxi Academy of Agricultural Sciences introduced the Bt insect resistance gene with my country's independent intellectual property rights into Jinmian 7 through the Agrobacterium-mediated method. As an insect-resistant gene donor (Tang Canming, Sun Jing et al. Genetic analysis of resistance to three ty...

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Abstract

The invention is a molecular-marking aided-decorating backcross-polymerization selecting-cotton method. Use Si-mian 3 in the Changjiang River valley as recurrent parent, and Shanxi 94-24 and 7235 series as anti-insect and good-quality aim-character supplying parent (non-recurrent parent), and respectively make them carry through hybridization and further backcross or self-cross for 2-3 generations, each of which adopts field observing method to comprehensively select agricultural character of recurrent parent, and adopts kanamycin fastness filtration, combination of anti-insect and detecting Bt gene's PCR detection and QTL molecule-marking aided-selection to respectively obtain destination middle material, and then make the material intercross, F1 generation self-cross aided with molecule-marking aided-selection to obtain new multiple-destination self-cross aided with molecular-marking

Description

[0001] (1) Technical field: a method for molecular marker-assisted modification of backcross polymerization and breeding of cotton varieties according to the present invention belongs to the breeding method of aggregating multiple cotton target traits, and is dedicated to rapid and effective breeding and production of multi-target traits in cotton new strains (new varieties). (2) Technical background: [0002] Recurrent selection is an effective means to break the negative correlation between yield, resistance and quality, and simultaneously improve yield, disease resistance and quality. As early as 1980, on the basis of summarizing domestic and foreign cotton breeding experience, Professor Pan Jiaju of our research institute combined the advantages of the two methods of hybridization and backcrossing between hybrid lines, and proposed a modified backcrossing that aggregates multiple excellent traits of cotton breeding law. The technical system of this method is to select an ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04C12Q1/68
Inventor 张天真郭旺珍朱协飞
Owner NANJING AGRICULTURAL UNIVERSITY
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