Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Efficient, broad-spectrum keratinase and its gene

A technology of keratinase and gene, applied in genetic engineering, plant genetic improvement, hydrolytic enzymes, etc., can solve problems such as high-efficiency strains that cannot be considered as genetic engineering

Inactive Publication Date: 2003-03-26
姚斌 +1
View PDF1 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although domestic research on keratinase was carried out earlier, they all focused on the isolation of strains. At present, a gene encoding keratinase has not been isolated, let alone the construction of highly efficient strains of genetic engineering.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Efficient, broad-spectrum keratinase and its gene
  • Efficient, broad-spectrum keratinase and its gene
  • Efficient, broad-spectrum keratinase and its gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] experiment one

[0017] This experiment illustrates the screening procedure for natural strains producing keratinase.

[0018] Feather keratin was used as the only N source, and keratinase-producing bacteria were screened by continuous enrichment culture. Rotten feather soil samples were diluted routinely and inoculated into the screening medium (1.5g / LKH 2 PO 4 , 0.025g / L MgSO 4 .7H 2 O, 0.025g / L CaCl, 0.015g / L FeSO 4 .7H 2 O, 0.005g / L ZnSO 4 , 0.5g / L feather meal, pH 6.0), cultured at 30°C for 10-15 days, picked the bacilli that propagated and grown in this culture medium and cultured them continuously for 3 rounds, then coated and separated on the screening medium plate, picked Take a single colony and re-inoculate it into the screening culture medium, and then repeat plate separation and liquid screening culture for the well-grown strains to purify the strains. 24 strains secreting keratinase were screened by this method.

[0019] In...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a keratinase and its coding gene. It has advantages of high activity, good heat stability, wide PH range and efficient degradation of alpha & beta-keratinase. It has also the character of common protease and can degrade many kinds of protein derived from plants and animals. It can be widely used in the trades such as feed, foods, washing, wool spinning and pharmacy.

Description

technical field [0001] The present invention relates to a kind of keratinase and its gene. technical background [0002] Keratinase is a class of enzymes that can hydrolyze keratin, and some keratinases can also hydrolyze various proteins except keratin. Keratin is characterized by highly interconnected cysteine ​​disulfide bonds, insoluble in water, salt, dilute acid and dilute alkali, and cannot be degraded by general proteases. Keratin is the main component of animal feathers, hair, and hooves, and is widely found in human and animal skin, scars, and scabs. [0003] Keratinase can be used in the feed industry to convert waste such as feathers and pig hair into high-nutrition feed protein. Keratin is the waste produced during poultry processing—the main component of feathers. The protein content in feather meal is as high as 90%, and the content of essential amino acids is extremely high. It is an excellent protein source in animal feed, but it contains cysteine Formed ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/50C12N15/57
Inventor 姚斌王亚茹操时树史秀云袁铁峥范云六
Owner 姚斌
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com