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Method of promoting haematococcus pluvialis growing multiplication and regulating astaxanthin synthesis and accumulation in photobiological reactor

A technology of photobioreactor and Haematococcus pluvialis, applied in biochemical equipment and methods, microorganisms, fermentation, etc., can solve the problems of light transmittance decline, biological pollution, low population density, etc., and achieve the regulation of synthesis and accumulation , high utilization rate of light energy, and the effect of promoting growth and proliferation

Inactive Publication Date: 2003-01-22
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main factors hindering the large-scale production of Haematococcus pluvialis are the high cost caused by the low population density of Haematococcus pluvialis culture process and the low yield per unit area.
Haematococcus pluvialis is cultivated in small-scale bioreactors or airlift bioreactors composed of pipeline photobioreactors or small immobilized systems. Decreased light transmittance and difficult to clean. Although different types of paddle wheels have been used to stir and mix to minimize the shading effect between algae cells and improve the utilization of light, it is only suitable for small-scale experiments in the laboratory.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Establish an all-weather closed large-scale operation system photobioreactor. The main reactor (water tank) is 500×150×65cm in length, width and depth. The bottom and surroundings are made of 2cm thick colorless transparent tempered glass, and the cover part is made of 0.2mm thick 20 pcs of 100W solar lamps and 20 pcs of 40W energy-saving spotlights with adjustable brightness are installed around and up and down the main reactor; the rotational speed of the speed-regulating paddle impeller agitator in the reactor is 30r / m; The rotation speed of the scroll wheel is 50r / m, and the brackish and fresh water nutrient solution circulates at a flow rate of 0.04m / s; the brackish and fresh water nutrient solution deployment and temperature control system is equipped with 3 liquid storage tanks with a capacity of 4000L, and the agitator rotates at a speed of 80r / m. The culture conditions for the growth and proliferation of algae cells and the regulation stage of increasing the pop...

Embodiment 2

[0022] Establish an all-weather closed large-scale operation system photobioreactor. The main reactor (water tank) has a length, width, and depth of 220×110×55cm. The bottom and surrounding areas are made of colorless and transparent special glass with a thickness of 1.5cm. The cover part is made of a thickness of 0.1 mm transparent film and sealed with adhesive tape, 20 40W fluorescent lamps with adjustable brightness are installed around and up and down the main reactor; the rotational speed of the speed-regulating paddle impeller in the reactor is 20r / m; the rotational speed of the water roll is 30r / m m, the brackish and fresh water nutrient solution circulates at a flow rate of 0.02m / s; the brackish and fresh water nutrient solution deployment and temperature control system is equipped with two liquid storage tanks with a capacity of 1200L, and the agitator speed is 60r / m. The culture conditions for the growth and proliferation of algae cells and the regulation stage of inc...

Embodiment 3

[0024] Establish an all-weather closed large-scale operation system photobioreactor. The main reactor (water tank) is 200×100×65cm in length, width and depth. The bottom and surrounding areas are made of 1cm thick colorless and transparent tempered glass, and the cover part is made of 0.2mm thick The transparent film is sealed with an adhesive, and the power of adjustable brightness is installed around the main reactor and up and down. The power is 7 40W fluorescent lamps and 5 100W solar lamps; The rotation speed is 10r / m, and the brackish and fresh water nutrient solution circulates at a flow rate of 0.03m / s; the brackish and fresh water nutrient solution deployment and temperature control system is equipped with 2 liquid storage tanks with a capacity of 800L, and the agitator speed is 20r / m. The culture conditions for the growth and proliferation of algae cells and the regulation stage of increasing the population density are as follows: the composition of salty and fresh wa...

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Abstract

The present invention relates to the process of promoting haemtococcus pluvialis growth, raising its population density and controlling the synthesis and accumulation of astaxanthin as intracellular secondary catabolite in a photobiological reactor system. The process includes establishing a photobiological reactor system with main reactor made of transparent material, outside controllable lighting facility, speed regulating vane wheel stirrer, water rolling wheel, bacteria-free ventilating and oxygen unit, salty and fresh water nutritious liquid compounding and temperature controlling unit, and cleaning and sterilizing unit; altering environment conditions to induce haemtococcus pulvialis to multiply, raise population density and regulating synthesis, accumulation and collecting haemtococcus pluvialis with rich astaxanthin.

Description

technical field [0001] The invention relates to a method for cultivating and promoting the growth and proliferation of Haematococcus pluvialis, increasing the population density, and regulating the synthesis and accumulation of intracellular secondary metabolite astaxanthin in an adjustable all-weather closed large-scale operation system photobioreactor. Background technique [0002] Haematococcus pluvialis is a single-celled green alga with oval or oval cells ranging in size from about 5 microns to 20-30 microns, sometimes even larger; it has two flagella of equal length , the swing of the flagella pushes the cells to swim freely; there is a gap between the cell wall and the protoplast, which is filled with gelatinous substances, and there is a cytoplasmic filament connection between the cell wall and the protoplast, and the vegetative reproduction is cell division, forming 2, 4, 8, 16 or even more Multiple daughter cells, asexual reprodu...

Claims

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Application Information

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IPC IPC(8): C12N5/02C12P23/00
Inventor 刘昕王江海袁建平黄晓霓
Owner SUN YAT SEN UNIV
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