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Prepn. of double-stranded nucleic acid on surface of solid support

A double-stranded nucleic acid and support technology, which is applied in sugar derivatives, organic chemistry, fermentation, etc., can solve the problems of reducing production costs, disadvantages, and high production costs, and achieve the goals of improving production efficiency, high progress, and revealing production costs Effect

Inactive Publication Date: 2003-01-08
SOUTHEAST UNIV
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Problems solved by technology

The disadvantage of this method is that longer oligonucleotides containing primer binding sites need to be synthesized on the surface of the solid phase medium, which is not conducive to reducing production costs
[0007] We have previously invented a double-stranded nucleic acid microarray preparation technology (National Patent Application No. of the People's Republic of China: 02112780.8), but its production cost is still relatively high,

Method used

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  • Prepn. of double-stranded nucleic acid on surface of solid support
  • Prepn. of double-stranded nucleic acid on surface of solid support
  • Prepn. of double-stranded nucleic acid on surface of solid support

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Embodiment Construction

[0021] 1. Preparation of solid support

[0022] The solid support for the preparation of double-stranded nucleic acid in the present invention can be LB film, (poly) tetrafluoroethylene film, (poly) vinylidene fluoride film, polystyrene film, chemical film such as polycarbonate, gel , surface chemically modified glass, silicon, gold and other materials used in solid-phase chemistry; the surface of the solid-phase support contains active groups such as carboxyl, aldehyde, amino, hydroxyl, thiol and other similar groups.

[0023] 2. Chemical synthesis of single-stranded oligonucleotides and preparation of single-molecule nucleic acid samples

[0024] Design and synthesis of probe single-stranded oligonucleotides (attached figure 2 A) and universal single-stranded oligonucleotides (attached figure 2 B), the two oligonucleotides were annealed after synthesis (attached figure 2 C), linking to form a single molecular oligonucleotide (attached figure 2 D).

[0025] 3. Immobi...

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Abstract

Manufacturing method of double-chain nucleic acid on surface of solid supporter is a distinctive method of manufacturing double-chain nucleic acid on solid supporter is basic molecule biology field and biomedicine field, the invention uses this method to manufacture solid supporter which connecting double-chain nucleic acid. The manufacturing method includes: (a) two single-chain few ribotide segments are chemical synthesized, one is universal single-chain few ribotide segment, the other is probe single-chain few ribotide segment, (b) in liquid reaction system the universal single-chain few ribotide segment and probe single-chain few ribotide are compounded in quality and carry on connecting enzyme connecting reaction, (c) single-molecular nucleic acid specimen is sampled onto the solid supporter's surface polished by aldehyde radicel.

Description

1. Technical field: [0001] The invention is a unique method for preparing double-stranded nucleic acid on a solid phase support in the fields of basic molecular biology and biomedicine. The method is used to prepare a solid phase support connected to double-stranded nucleic acid. 2. Background technology: [0002] The technology of assembling double-stranded nucleic acid on a solid support has important application value in the fields of manufacturing double-stranded nucleic acid microarray chips, screening nucleic acid binding proteins (such as transcription factors) and nucleic acid binding drugs. In particular, the present invention provides an economical and efficient technical system for preparing double-stranded nucleic acid microarray chips. [0003] Biochips mainly refer to microarrays composed of nucleic acids, proteins, cells, micro-tissues and other biologically active substances assembled on solid supports. The application of biochips can realize the accurate, r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H21/04C12P19/34
Inventor 王进科白云飞陆祖宏
Owner SOUTHEAST UNIV
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