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Acid resistant saccharomycete and its method of biological removing heavy metal in sudge

A technology of yeasts and heavy metals, applied in the field of environmental engineering, can solve problems such as being unable to be effectively removed, and achieve the effects of promoting growth activity and acid production, mild reaction, and no secondary pollution

Active Publication Date: 2007-05-09
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the main toxic heavy metal Cr in the sludge cannot be effectively removed, and its removal rate is only 13-57%.

Method used

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  • Acid resistant saccharomycete and its method of biological removing heavy metal in sudge

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Sampling

[0032] Take the sludge from the thickening tank of a tannery wastewater treatment plant in Zhejiang. The basic properties of the tannery sludge are shown in Table 1.

[0033] pH

Cr

mg / kg

Solid content

N

P

organic matter

％

8.21

14730

4.84

2.83

0.81

45.68

[0034] (2) Cultivation of yeast R30

[0035]Prepare PDA medium, 200g of potatoes, 20g of sucrose or glucose, 1000mL of water, natural pH, sterilized at 121°C for 30min. Liquid culture conditions: take the yeast R30 lawn from the slant of the PDA medium, inoculate it in the PDA liquid medium, cultivate it on a reciprocating shaker at 180rpm for 2 days at 28-30°C, and the number of bacteria reaches 10 8 individual / mL.

[0036] (3) Thiobacillus strains

[0037] Thiobacillus thiooxidans TS 6 , is a strain preserved in our laboratory, which has been preserved in the General Microbiology Center of China Mi...

Embodiment 2

[0044] (1) Sampling: Take the sludge from the thickening tank of a sewage treatment plant in Jiangsu. The basic characteristics of the sludge are shown in Table 3.

[0045] pH

containing solid

Rate

N

P

organic matter

Zn

Cr

Cu

％

mg / kg

7.56

3.34

2.69

1.15

43.7

1578

310

522

[0046] (2) the cultivation of yeast R30, with embodiment 1

[0047] (3) Thiobacillus thiooxidans TS 6 Bacterial strain: with embodiment 1

[0048] (4) Biological removal of Zn, Cr, and Cu from municipal sludge

[0049] Add 5g / L elemental sulfur to municipal sludge and inoculate with 10% Thiobacillus thiooxidans TS 6 , 3% Rhodotorula sp.R30, 180rpm shaking culture treatment in a shaker for 4 to 6 days; a control treatment without Rhodotorula sp.R30 was set up, and the pH change in the reaction medium was measured every day. After the rea...

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Abstract

An acid-resistant rhodotorula R30 (CGMCC No.1147) for removing heavy metals from sludge is disclosed. The process for removing heavy metals from sludge includes loading the sludge in bioreactor, inoculating said rhodotorula R30 and thiobacillus TS6, aerating at 28 deg.C for 2-6 days, dewatering of sludge, regulating pH of liquid phase for depositing heavy metals, and neutralizing solid phase for agricultural purpose.

Description

1. Technical field [0001] The invention relates to an acid-resistant red yeast and its method for biological removal of heavy metals from sludge. It is a technology for removing heavy metals in municipal sludge and industrial sludge by using the interaction between yeast and sulfur bacteria, and belongs to environmental engineering. technology field. 2. Technical background [0002] Every year, a large amount of various heavy metal-containing industrial sludge and urban municipal sludge are produced in my country and urgently need to be disposed of safely. Although land use of sludge has been considered as a way of low disposal cost and resource utilization of sludge for many years, due to the presence of toxic heavy metals in sludge (Cu, Zn, Cr, Ni, etc. Heavy metal content can reach hundreds to thousands of mg / kg, Cr content in industrial sludge such as tannery sludge can reach 1% to 4%; heavy metal in electroplating sludge can reach 10% or even higher), sludge land use ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C02F3/34C02F1/62C12R1/85
Inventor 周立祥王世梅
Owner NANJING AGRICULTURAL UNIVERSITY
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