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Method for extracting deoxyribonucleic acid from edible oil

A technology of deoxyribonucleic acid and extraction method, which is applied in the field of extracting deoxyribonucleic acid from edible oil, and can solve the problems of cumbersome methods and the like

Inactive Publication Date: 2007-04-18
北京陆桥技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] This shows that the method mentioned in this prior art is comparatively loaded down with trivial details, therefore be necessary to provide a kind of simple, fast, effective method for extracting DNA from edible oil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 30ml of edible oil sample into a 50ml centrifuge tube, add 15ml of water, shake vigorously to mix, centrifuge at 12000r / m for 15min; take the lower layer of water and transfer it to another 50ml centrifuge tube, add 2.5 times the volume of absolute ethanol, and gently invert Mix well, and centrifuge at 12000r / m for 20min at 4°C; discard the supernatant, add 500μl of 70% ethanol to wash the precipitate twice, and centrifuge at 12000r / m for 20min to obtain DNA.

Embodiment 2

[0023] Take 15ml edible oil sample into a 50ml centrifuge tube, add 10ml TE buffer [10mmol / LTris-HCl (pH 8.0), 1mmol / L EDTA (pH 8.0)], shake vigorously and mix well, centrifuge at 12000r / m for 15min; remove the lower layer Transfer the water phase to another 50ml centrifuge tube, add 1.5 times the volume of absolute ethanol, gently invert and mix well, centrifuge at 12000r / m, 4°C for 20min; discard the supernatant, add 500μl 70% ethanol to wash the precipitate twice, 12000r / m centrifugation for 20min to obtain DNA.

Embodiment 3

[0025] Take 50ml of edible oil sample into a 100ml centrifuge tube, add 25ml of potassium phosphate buffer (100mmol / L, pH7.5), shake vigorously and mix well, centrifuge at 12000r / m for 15min; take the lower layer of water and transfer it to another 100ml centrifuge tube , add 0.8 times the volume of isopropanol, gently invert and mix well, centrifuge at 12000r / m for 20min at 4°C; discard the supernatant, add 500μl of 70% ethanol to wash the precipitate twice, centrifuge at 12000r / m for 20min to obtain DNA.

[0026] Compared with the prior art, the invention has the advantages that the DNA can be extracted from the edible oil simply, quickly and efficiently.

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Abstract

The present invention is the process of extracting NDA from edible oil. The process includes adding water or other buffering liquid into edible oil, mixing via intense vibration and centrifuging; taking lower water phase before adding anhydrous alcohol and isopropanol, mixing and centrifuging; and twice washing of deposit with ethanol. The said process is suitable for extracting DNA from edible oil with low DNA content, and the extracted NDA is suitable for PCR proliferation and detection.

Description

technical field [0001] The invention belongs to the technical field of separation by physical or chemical methods, relates to a method for extracting required components from edible oil, in particular to a method for extracting deoxyribonucleic acid (DNA) from edible oil. Background technique [0002] Deoxyribonucleic acid (DNA) is genetic material. By analyzing and detecting DNA, genes and their biological species can be identified. Edible oil is processed under a series of special physical conditions such as high temperature, and the DNA is severely damaged. It is difficult to extract enough DNA from the edible oil, so it is also difficult to identify the variety or strain of the raw material for edible oil production. [0003] The prior art more related to the present invention is "Qualitative Detection of Genetically Modified Components in Edible Oils and Fats by PCR Method" published in the 2nd issue of "China Oils and Fats" in 2002. This document mentions the extracti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H21/04C07H1/08
Inventor 徐宝梁刘博陈颖王丙武王曙光苏宁
Owner 北京陆桥技术股份有限公司
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