Method for preparing tea pigment

A technology for tea pigments and tea polyphenols, which is applied in the field of oxidizing tea polyphenols to generate tea pigments, can solve the problems of complicated processes, low yield of theaflavins, etc., and achieves improved yield and purity, mild reaction and easy control, and good dissolution. sexual effect

Inactive Publication Date: 2007-03-14
SHIJI BIOLOGICAL MEDICINES WUXI CITY
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Problems solved by technology

[0003] Aiming at the disadvantages of low yield of theaflavins and cumbersome process in the above method for producing theaflavins, the present invention provides another production method of theaflavins with high yield of theaflavins, optimized process and low production cost

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  • Method for preparing tea pigment

Examples

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Embodiment 1

[0016] Example 1: Put 100Kg of pre-cooled fresh tea leaves freshly picked into a reaction kettle after crushing them with a masher, add 100Kg of tea polyphenols, then add 500L of ethyl acetate solution, and then add acetic acid with pH=6.5 500L aqueous solution, ventilated and stirred for 300 minutes at 65°C, cooled to 15°C, kept warm for 1 hour, separated the organic phase, added water with a ratio of 1:0.5, stirred thoroughly for 20 minutes, and then centrifuged to obtain the organic phase. Send it to a scraper concentrator with a temperature of 60°C and a vacuum of 0.06Mpa to concentrate to a paste, and then send it to a vacuum oven with a temperature of 45°C and a vacuum of 0.06Mpa to dry to powder. The yield of thea pigment is 70%. According to the Roberts method detection: theaflavin is 45%, thearubigin is 4.5%, and theabrownin is 3.3%.

Embodiment 2

[0017] Example 2: Put 100Kg of pre-cooled fresh tea leaves freshly picked into the reaction kettle after crushing them with a masher, add 10Kg of tea polyphenols, then add them to 500L of ethanol solution, and then add pH=5 citric acid aqueous solution 50L, ventilate and stir at 40°C for 240 minutes, cool down to 15°C, keep warm for 1 hour, separate the organic phase, add water at a ratio of 1:1, stir thoroughly for 20 minutes, then centrifuge to obtain the organic phase, send Concentrate to a paste in a scraper concentrator with a temperature of 62°C and a vacuum of 0.07pa, and then send it to a vacuum oven with a temperature of 50°C and a vacuum of 0.07Mpa to dry to powder. The yield of tea pigment is 70%, and according to Roberts method detection: theaflavin is 60%, thearubigin is 4.5%, theabrown is 2.8%.

Embodiment 3

[0018] Embodiment 3: 100Kg of pre-cooled fresh tea leaves freshly picked are crushed with a masher and then put into the reactor, add tea polyphenols 33Kg, polysaccharide hydrolase 500g, then add in 660L of isopropanol solution, and then add 220L of citric acid aqueous solution with pH=6, ventilated and stirred at 30°C for 180 minutes, cooled to 15°C, kept warm for 1 hour, separated the organic phase, added water in a ratio of 1:2, stirred thoroughly for 20 minutes, and cooled to 15°C ℃, keep warm for 1 hour, and then centrifuge to obtain the organic phase, send it to a scraper concentrator with a temperature of 63°C and a vacuum of 0.08Mpa to concentrate to a paste, and then send it to a temperature of 55°C and a vacuum of 0.08Mpa Dry to powder in a vacuum oven. The yield of thea pigment was 78%. According to the Roberts method detection: theaflavin was 35%, thearubigin was 5.1%, and theabrownin was 3.1%.

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Abstract

A process for preparing the tea pigment includes such steps as mashing fresh tea leaves, adding enzyme, adding them to the solution of theopolyphenol in organic solvent, adding acidic aqueous solution, enzymatic oxidizing-polymerizing reaction, separating to obtain tea red, tea yellow and tea brown, purifying, concentrating and drying.

Description

(1) Technical field [0001] The invention relates to a method for producing tea pigment by using tea extract, specifically, a method for oxidizing tea polyphenols to produce tea pigment by using the enzyme of tea itself with or without adding enzyme purchased from the market. (2) Background technology [0002] Tea pigment is a mixture of polyphenols and their derivatives in tea, and its main components are theaflavins, thearubigins, and theabrownins. Among them, theaflavins have the strongest pharmacological activity. At present, the international demand for tea pigments is mainly based on the content of theaflavins. On February 28, 2001, the Chinese Patent Office announced a high-activity tea pigment and its production. The method, the application number is 00112536, is characterized in that the fresh tea leaves are directly mashed into slag and leaves, and then a buffer solution is added, with or without the addition of tea extract tea polyphenols, and the enzymatic oxidati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/06
Inventor 吴心南于静波赵福林徐尧
Owner SHIJI BIOLOGICAL MEDICINES WUXI CITY
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