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Process for raising producing rate of taxad alcohol of Taxus chinensis endogeny fungus fermenting material

A technology of endophytic fungi and paclitaxel, applied in the direction of fermentation, fungi, etc., can solve the problem of low fermentation yield

Inactive Publication Date: 2007-03-14
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These fermentation production levels are very low, and there is still a certain gap from industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] In this example, two endophytic fungi XT1 (high-yielding paclitaxel synthesis precursors 10-deacetylbacatin III and baccatin III, but almost no paclitaxel production) and XT5 (taxol production) were selected in this example. ) Is mixed culture to produce paclitaxel.

[0034] The identification of the two endophytic strains involved in this example (Fungi Identification Manual, Wei Jingchao, Shanghai Shanghai Science and Technology Press, 1979, 645-649) is as follows:

[0035] Morphological characteristics, products and taxonomic status of endophytic fungi

[0036] Strain

Codename

Host plant

Product

Morphological characteristics

Classification status

XT1

Taxus chinensis

Taxus chinnsis

Var.mairei

10- go to B

Acylbacatin

III and Bar

Catin III

Only infertile hyphae, no sexual and

Asexual spores, mycelium like cotton wool,

No sclerotium, thick hyphae, intertwined

Reticulate, white in the early s...

Embodiment 2

[0057] Change the sucrose in the fermentation medium in Example 1 to 10g / L, phenylalanine to 1mg / L, sodium acetylsalicylate to 0.5mg / L, oleic acid to 10ml / L, and other conditions remain unchanged. XT5 and XT1 were mixed culture, XT5 and XT1 were cultured separately, and the content of paclitaxel in the fermentation was 1094.0274μg / L, 276.7554μg / L, 0μg / L, respectively.

[0058] Instance number

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PUM

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Abstract

A method for increasing the output rate of taxol in the endofungus fermented substance of enqlist yew features that the endofungus generating taxol and the synthesized precursors 10-deacetyl Bakating C and Bakating C able to generate taxol are individually cultured, and the cultured substances are then mixed and cultured while additional culture medium is added. As a resultant the output rate of taxol is greatly increased.

Description

Technical field [0001] The method is a method that uses a taxol-producing endophytic fungus culture and a taxol-producing synthetic precursor endophytic fungal culture for mixed culture to greatly increase the paclitaxel content in the fermentation, and belongs to the field of biotechnology. Background technique [0002] Since wani et al. extracted paclitaxel from the bark of Taxus brevifolia Nutt. and discovered that it has anti-cancer effects, researchers have successively discovered its unique anti-cancer mechanism and can treat many kinds of cancer. Therefore, people began to extract paclitaxel from the bark of yew in large quantities to meet the needs of the pharmaceutical market. Since the yew tree is a rare plant in the world, extracting paclitaxel from the bark will inevitably severely destroy natural resources and endanger the ecological balance. In order to solve the shortage of medicine sources, scholars at home and abroad have done a lot of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P17/02
Inventor 谈锋胡凯祝顺琴唐克轩廖志华
Owner SOUTHWEST UNIV
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