Extract of cocoa tea in application for preparing medication of foodstuff of preventing and improving fatigue and clinical symptom

A technology of clinical symptoms and extracts, which is applied in the application field of cocoa tea extracts, can solve the problems of anti-fatigue activity of cocoa tea, etc., and achieve the effects of improving physical strength, improving depression, and alleviating anxiety

Inactive Publication Date: 2007-01-31
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although cocoa tea is a close relative of traditional tea, the anti-fatigue activity of cocoa tea has not been explored in any applications

Method used

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  • Extract of cocoa tea in application for preparing medication of foodstuff of preventing and improving fatigue and clinical symptom
  • Extract of cocoa tea in application for preparing medication of foodstuff of preventing and improving fatigue and clinical symptom
  • Extract of cocoa tea in application for preparing medication of foodstuff of preventing and improving fatigue and clinical symptom

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Take 100g of dried cocoa tea leaves, add 2000ml of 90°C hot water to extract for 4 minutes. After the extract is filtered through gauze, the filtrate is centrifuged at 1000 rpm for 5 minutes, and the supernatant is recovered and freeze-dried. The above freeze-dried extract of cocoa tea leaves was used in the following examples of the present invention.

Embodiment 2

[0040] Embodiment two: HPLC analysis of cocoa tea extract

[0041] The above cocoa tea extract was prepared into a 1% aqueous solution, filtered through Sep-Pakt C18 Cartridge (5 ml, manufactured by Waters), washed with water, and then eluted and separated with acetonitrile. Subsequently, 250 mg of the resulting separated fraction was washed on a Develosil C30-UG-5 (20 mm × 250 mm, manufactured by Nomura Chemical) column with 5-30% acetonitrile in the presence of 0.05% trifluoroacetic acid (TFA) in a linear gradient. The way of off (5ml / mim, 180 minutes, 220nm ultraviolet detection) elution separation. Recover the separated components obtained, and then use a YMC-Pak ODS (20×250mm, manufactured by YMC) column, in the presence of 0.1% TFA, use 20-25% acetonitrile in a linear gradient elution mode (6ml / mim, 60 minutes, 280nm ultraviolet detection) elution purification. The result is as figure 1 As shown, three components different from traditional tea were separated from it. ...

Embodiment 3

[0064] Example 3: Mouse spontaneous movement test (ambulatory activity test)

[0065] 7-week-old male ICR mice (purchased from CLEA Japan) were used. Mouse at room temperature (23±1~2℃), humidity 55±5%, ventilation times 12~15 times / hour, lighting time 12 hours / day (turn on the light at 7 am and turn off the light at 7 pm); solid Feed: CE-2 (CLEA company); the test was carried out after preparatory feeding for 1 week under the feeding condition of free drinking water. The present invention uses a laboratory animal exercise measurement device (LaboratoryAmbulometer MODEL SMA-10 O'HARA & Co., Ltd. (Quantitative analysis of the effects of lithium on the reverse tolerance and the c-Fos expression induced by methamphetamine in mice. Brain Research Protocols.4 : 11-18, 1999.)) Determination of spontaneous exercise in mice. The mice were placed in the experimental device to adapt to the experimental environment 30 minutes before the administration of the specimen, and the total amo...

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PUM

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Abstract

An application of cocao extract and its active component in preparing the medicines and food for preventing and treating exhaustion syndrome and its clinic symptons is disclosed.

Description

technical field [0001] The invention relates to the field of natural plant extraction and application, in particular to the application of cocoa tea extract. Background technique [0002] Today, with the rapid development of material civilization and spiritual civilization, while people are constantly accepting new things, they are also feeling and bearing various new spiritual pressures that have never been seen in the past. Some people exaggerate the generalization: the modern society of knowledge explosion is an era of mental stress, in fact, this statement is not too much. Generally speaking, modern people's fatigue mostly refers to mental stress or mental fatigue. In the past, as a general term, fatigue mostly refers to physical and mental states such as fatigue, burnout, and loss of appetite, as well as a broad generalization of various clinical symptoms such as sleep disorders and low motivation caused by mental fatigue. Traditional anti-fatigue focuses on improving...

Claims

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Application Information

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IPC IPC(8): A61K36/82A61P43/00A61K127/00A23L33/105A61P25/00C07H1/08
Inventor 栗原博姚新生叶创兴
Owner JINAN UNIVERSITY
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