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New technology of high effect extracting purified astaxanthin from Fafu yeast

A technology of Phaffia yeast and astaxanthin, applied in organic chemistry and other directions, can solve the problem of easy oxidation of extracts, and achieve the effect of short cycle, high purity of finished products, and simplified purification steps.

Inactive Publication Date: 2005-09-21
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention also uses a self-made nitrogen protection cabinet to solve the problem that the extract is easily oxidized during the operation and simplifies the purification steps

Method used

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  • New technology of high effect extracting purified astaxanthin from Fafu yeast

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Mix 500ml of Phaffia fresh material with 5000ml of 90% ethanol solution, perform negative pressure cavitation and swirl solid-liquid extraction for 40 minutes, centrifuge to retain the supernatant, add 5000ml of 90% ethanol solution to the remaining solids, Negative-pressure cavitation mixed-rotation solid-liquid extraction for 40 minutes, centrifuge to retain the supernatant, add 5000ml of 90% ethanol solution to the remaining solids, negative-pressure cavitation mixed-rotation solid-liquid extraction for 40 minutes, centrifuge to discard the precipitate, and three times The supernatants obtained by centrifugation were combined and then concentrated to 150ml under reduced pressure. The pressure was -0.06--0.1Mpa and the temperature was 50°C. The melting time is 5 minutes, the air flow rate is 2-8 cubic meters per hour, and the pressure is -0.06--0.1Mpa. After standing still, slowly add distilled water to promote stratification, take the lower methylene chloride layer an...

Embodiment 2

[0024] Mix 2L of the fresh material of Phaffia yeast with 30L of 85% ethanol solution, carry out negative pressure cavitation and swirling solid-liquid extraction for 1 hour, centrifuge to retain the supernatant, add 30L of 85% ethanol solution to the remaining solids, Negative-pressure cavitation mixed-rotation solid-liquid extraction for 1 hour, centrifuge to retain the supernatant, add 30L of 85% ethanol solution to the remaining solids, negative-pressure cavitation mixed-rotation solid-liquid extraction for 1 hour, centrifuge to discard the precipitate, and three times The supernatants obtained by centrifugation were combined and concentrated under reduced pressure to 900ml. The pressure was -0.06--0.1Mpa and the temperature was 50°C. The melting time is 4 minutes, the air flow rate is 2-8 cubic meters per hour, and the pressure is -0.06--0.1Mpa. After standing still, slowly add distilled water to promote stratification, take the lower methylene chloride layer and wash it w...

Embodiment 3

[0026] Mix 120g of Phaffia dry matter with 2.4L of 90% ethanol solution, negative pressure cavitation and swirling solid-liquid extraction for 1 hour, centrifuge to retain the supernatant, add 2.4L of 90% ethanol to the remaining solid Solution, negative pressure cavitation mixed rotation solid-liquid extraction for 1 hour, centrifuge to retain the supernatant, add 2.4L of 90% ethanol solution to the remaining solids, negative pressure cavitation mixed rotation solid-liquid extraction for 1 hour, centrifuge to discard the precipitate , Combine the supernatants obtained by centrifuging three times and concentrate them under reduced pressure to 500ml. The pressure is -0.06--0.1Mpa and the temperature is 50°C. Liquid extraction, the cavitation time is 6 minutes, the air flow rate is 2-8 cubic meters per hour, the pressure is -0.06--0.1Mpa, after standing still, slowly add distilled water to promote stratification, take the lower methylene chloride layer and wash it with water for ...

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Abstract

A process for effectively extracting astaxanthin from Fafu yeast and purifying it includes proportionally mixing raw material with menstruum, negative-pressure cavitation vortex solid-liquid extraction, centrifugal separation to obtain liquid extract, vacuum concentrating, negative-pressure cavitation vortex extracting by solvent, washing the liquid extract by water, dewatering, vacuum concentrating, negative-pressure column chromatography and positive-pressure column chromatography. The purity of product is 98%.

Description

Technical field [0001] The invention relates to a method for efficiently extracting and purifying astaxanthin from Phaffia yeast. Background technique [0002] Astaxanthin 3,3′-dihydroxy-4,4′-diketone-β,β′-carotene, molecular formula C 4 Oh 52 o 4 , with a molecular weight of 596.86, is a terpene-like unsaturated compound, pink in color, and is one of more than 600 carotenoids. Astaxanthin is easy to oxidize and becomes astaxanthin after oxidation. The structural formula of astaxanthin is shown in Figure 1: [0003] [0004] The structural formula of Fig. 1 astaxanthin [0005] Natural astaxanthin is a high-quality, efficient and safe coloring agent and natural antioxidant. As a new type of feed additive, it can improve the immunity and survival rate of animals, and increase the nutritional value and appearance color of animal products. [0006] At present, in the extraction and purification of domestic astaxanthin, the primary problem to be solv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C403/24
Inventor 祖元刚薛艳华史权付玉杰唐中华刘莉娜李庆勇祖柏实
Owner NORTHEAST FORESTRY UNIVERSITY
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