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Method of regulating haematococcus pulvialis population density and synthesizing and accumulating astaxanthin by photoreactor

A photobioreactor and Haematococcus technology, applied in biochemical equipment and methods, microorganisms, fermentation, etc., can solve the problems of decreased light transmittance, hindering the large-scale production of Haematococcus, and low yield per unit area

Inactive Publication Date: 2004-12-22
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the main factor hindering the large-scale production of Haematococcus is the high cost caused by the low population density of Haematococcus during the cultivation process and the low yield per unit area.
Haematococcus cultured in a small-scale bioreactor closed system composed of a pipeline photobioreactor or a small immobilized system or an airlift bioreactor, the defect is that the pipe wall is easily covered by algal cell adhesion, resulting in Decreased light transmittance and difficult to clean. Although different types of paddle wheels have been used to stir and mix to minimize the shading effect between algae cells and improve the utilization of light, it is only suitable for small-scale experiments in the laboratory.

Method used

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Examples

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Effect test

Embodiment 1

[0021] An open large-scale operation system photobioreactor modified and optimized on the basis of a shallow water channel photobioreactor system. The main reactor (water tank) is 500×150×45cm in length, width and depth, and the bottom and sides are 2cm thick. Colorless transparent tempered glass, the main reactor up and down and side equalization device with adjustable brightness power of 30 100W solar lamps, 20 40W energy-saving spotlights; the speed of the speed-regulating paddle impeller agitator in the reactor is 30r / m; The rotation speed of the water wheel is 60r / m; the storage tank capacity of the nutrient solution and temperature control system is 4000L, and the rotation speed of the agitator is 80r / m. The composition of nutrient solution: 45% of clear water, 55% of sea water, potassium nitrate, sodium acetate and other nutrient salts are adjusted according to the physiological needs of different growth stages. Clean water and sea water can be sterilized by the ozone g...

Embodiment 2

[0023] An open large-scale operation system photobioreactor modified and optimized on the basis of shallow waterway photobioreactor system. The main reactor (water tank) is 220×110×35cm in length, width and depth, and the bottom and sides are 1.5cm Thick colorless and transparent tempered glass, the upper and lower sides of the main reactor and the equalizing device on the side can adjust the brightness and the power is 12 40W fluorescent lamps; the speed regulating paddle impeller agitator speed in the reactor is 20r / m; ; The capacity of the storage tank of the nutrient solution and temperature control system is 1000L, and the speed of the agitator is 60r / m. Nutrient solution composition: 30% clear water, 70% seawater, potassium nitrate, sodium acetate and other nutrient salts are adjusted according to the physiological needs of different growth stages. Clean water and sea water can be sterilized by the ozone generator. The optimized culture conditions for the pure culture o...

Embodiment 3

[0025] An open large-scale operation system photobioreactor modified and optimized on the basis of a shallow water channel photobioreactor system. The main reactor (water tank) is 200×100×35cm in length, width and depth, and the bottom and sides are 1cm thick. Colorless and transparent tempered glass, the main reactor's upper and lower and side equalization devices have adjustable brightness power of 9 40W fluorescent lamps; the speed regulating paddle impeller agitator speed in the reactor is 10r / m; the water roll speed is 10r / m; The liquid storage tank of the nutrient solution and temperature control system has a capacity of 800L, and the rotational speed of the agitator is 20r / m. Nutrient solution composition: 55% clear water, 45% seawater, potassium nitrate, sodium acetate and other nutrient salts are adjusted according to the physiological needs of different growth stages. Clean water and sea water can be sterilized by the ozone generator. The optimized culture condition...

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Abstract

The present invention relates to the process of regualting haemtococcus pluvialis population density and synthesizing and accumulating astaxanthin as intracellular secondary catabolite in a photobiological reactor system. The process includes establishing a photobiological reactor system with main reactor made of transparent material, outside controllable lighting facility, speed regulating vane wheel stirrer, water rolling wheel, ventilating and oxygen supplying unit, nutritious liquid compounding and temperature controlling unit and sterilizing unit; antering environment conditions to regulate haemtococcus pluvialis population density and synthesize, accumulate and collect astaxanthin; low temperature drying; etc. The natural astaxanthin is a kind of carotinoid with important physiological functions and very strong antioxidant activity.

Description

technical field [0001] The invention relates to a method for adjusting the population density of Haematococcus and the synthesis and accumulation of intracellular secondary metabolite astaxanthin by an optimized open-type large-scale operation system photobioreactor. Background technique [0002] Haematococcus (Ag.) is a single-celled micro-green algae, the cells of which are about 19-63 microns. Haematococcus motile cells are oval or ovoid with two equal-length flagella. There is a gap between the cell wall and the protoplast, which is filled with gelatinous substances. The cell wall and the protoplast are connected by cytoplasmic filaments. Vegetative reproduction is cell division to form 2, 4, 8 or more daughter cells. Asexual reproduction forms gelatin when the environment is unfavorable. Groups or thick-walled spores, sexual reproduction is isogamous. It is green under normal growth conditions, and the pigments contained in its cells ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/02C12P23/00
Inventor 刘昕袁建平陈峰王江海黄晓霓
Owner SUN YAT SEN UNIV
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