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Extracting and purifying method for chlorogenic acid in honeysuckle

A purification method, the technology of honeysuckle, applied in the direction of chemical instruments and methods, organic chemistry, carboxylate preparation, etc., can solve the problems of low yield and purity of extracts, and achieve a simple method, high purity, and high yield Effect

Inactive Publication Date: 2004-12-22
CHINA AGRI UNIV
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Problems solved by technology

[0003] The object of the present invention is to provide a method for extracting and purifying chlorogenic acid from Flos Lonicerae, solving the problem of low yield and purity of the extract

Method used

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  • Extracting and purifying method for chlorogenic acid in honeysuckle
  • Extracting and purifying method for chlorogenic acid in honeysuckle

Examples

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Embodiment Construction

[0010] specific implementation plan

[0011] Example 1: See the attached picture, take 1kg of honeysuckle, break it into small pieces of about 5mm long, add 10L of 70% ethanol to reflux extract for 2 hours, filter, add 70% ethanol to 8L of reflux to extract for 1 hour, filter, and combine twice The filtrate was concentrated under reduced pressure, ethanol was reclaimed, concentrated to about 2kg, 1% chitosan solution was added to the concentrated solution, stirred for 15 minutes and allowed to stand, the supernatant was filtered and the pH value was adjusted to be neutral, passed through AB-8 For macroporous resin, take the effluent, wash the column with water 3 times of the supernatant, combine the washing liquid and the effluent, concentrate, dry and pulverize. The yield of the product chlorogenic acid was 22%, and the purity was 48% (determined by spectrophotometry).

[0012] Example 2: See the attached picture, take 100g of honeysuckle, break it into small pieces about 3m...

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Abstract

The present invention relates to extraction and purification of functional components in Chinese herbal medicine and aims at provindg extraction and purification process of chlorogenic acid in honeysuckle in relatively high yield and purity. The techonlogical scheme includes twice reflux extraction of honeysuckle cut into sections with dilute alcohol, concentrating the extractive, flocculating for initial purification and final purification through macroporous resin or ethyl acetate extraction. The said process is simple and practical and has less loss of chlorogenic acid and high product yield and purity.

Description

technical field [0001] The invention relates to a method for extracting and purifying functional components of Chinese herbal medicines, in particular to a method for extracting and purifying chlorogenic acid from honeysuckle. Background technique [0002] Honeysuckle is the dried flower bud of Lonicerae Lonicerae, and it is one of the traditional Chinese medicines commonly used in clinic. Chlorogenic acid is the main active ingredient of honeysuckle, which has significant heat-clearing and detoxifying, anti-bacterial, anti-inflammatory and anti-aging effects, and has a significant inhibitory effect on digestive tract cancer. In addition to medicinal use, it can also be used for food and fruit preservation, and has the functions of increasing flavor and protecting color. Chlorogenic acid is an organic acid containing carboxyl and o-diphenolic hydroxyl groups, and is easily soluble in solvents such as water, alcohol, and acetone. At present, the methods for extracting chlor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C67/58C07C69/732
Inventor 韩鲁加阎巧娟江正强
Owner CHINA AGRI UNIV
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