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Nerve stem cell culture medium and its preparation method

A technology of neural stem cells and culture medium, applied to the culture medium of neural stem cells, the field of preparation of the neural stem cell culture medium

Inactive Publication Date: 2004-11-17
姜晓丹 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main function of these synthetic media is to provide a general survival and growth microenvironment for different types of tissue cells, but the above-mentioned cell culture media lack the ability to differentiate seed cells from different sources (such as bone marrow tissue cells, etc.) into neural stem cells.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The present embodiment one is made up of the following components by weight ratio:

[0030] 12g of basal culture medium mixed with DMEM and F12 at a ratio of 1:1

[0031] Insulin 5mg

[0032] L-Glutamine 3.9mg

[0033] Butylenediamine Hydrochloride (Putrescine) 10mg

[0034] Sodium Selenide 0.03mg

[0035] Human transferrin (Transferrin) 100mg

[0036] Hydrocortisone 10mg

[0037] Progesterone 0.02mg

Embodiment 2

[0038] Present embodiment two is made up of the following components by weight ratio:

[0039] 10g of basal culture medium mixed with DMEM and F12 at a ratio of 1:1

[0040] Insulin 4.5mg

[0041] L-Glutamine 3.5mg

[0042] Butylenediamine Hydrochloride (Putrescine) 9.2mg

[0043] Sodium Selenide 0.01mg

[0044] Human transferrin (Transferrin) 50mg

[0045] Hydrocortisone 5mg

[0046] Progesterone 0.01mg

Embodiment 3

[0047] Present embodiment three is made up of the component of following weight ratio:

[0048] 25g of basal culture medium mixed with DMEM and F12 at a ratio of 1:1

[0049]Insulin 12.5mg

[0050] L-Glutamine 12.5mg

[0051] Butylenediamine hydrochloride (Putrescine) 35.6mg

[0052] Sodium Selenide 0.51mg

[0053] Human transferrin (Transferrin) 150mg

[0054] Hydrocortisone 35mg

[0055] Progesterone 0.55mg

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Abstract

The invention discloses nervous stem cells substrate and the preparation method of it, it is made up of base cultivate liquid which is mixed of DMEM and F12 by rate of 1:1, and insulin, muriatic acid butyl diamine, selenium natrium, hydrogen cortisone, L-glutamic acyl amic man-turn iron albumen, flavone. The invention has function that seed cells of different sources such as narrow organize can be directionally developed and divided into nervous stem cells, it can provide required correlative nervous stem cells momentarily and inerrably to iatrical scientific research and teaching and clinic applicatino. Its confecting method is simple, it also have better repetition and simple and feasible operation.

Description

technical field [0001] The invention relates to a cell culture medium, in particular to a culture medium for culturing neural stem cells, and also relates to a preparation method of the neural stem cell culture medium. Background technique [0002] Neural stem cell research is a new research topic at home and abroad since 1998. Neural stem cells are pluripotent cells of the central nervous system and common precursor cells of the three main cells in the brain (neurons, astrocytes, and oligodendrocytes), and have the following characteristics: (1) in an undifferentiated state (2) have multilineage differentiation potential, that is, the ability to evolve into different types of mature cells; (3) can self-replicate or renew through asymmetric division, and produce the same offspring as itself cells to maintain a stable cell number. There are four main sources of neural stem cells: embryonic stem cells (embryonic stem cells, ES cells) or embryonic neural tissue, neural crest ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00
Inventor 姜晓丹徐如祥
Owner 姜晓丹
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