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High-efficient production method for 10-hydroxycamptothecine

A technology of hydroxycamptothecin and its production method, applied in the fields of seed and rhizome treatment, organic chemistry, application, etc., can solve the problems of complex extraction and separation process, low product yield, long production cycle, etc., and achieve simple process flow, The effect of high extraction rate and short production cycle

Inactive Publication Date: 2004-10-27
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The above-mentioned process, although in-depth research has been carried out on the raw materials for extracting 10-hydroxycamptothecin and the method of biotransformation synthesis, as well as the use of physical methods to induce the biotransformation of camptothecin in vivo to 10-hydroxycamptothecin, However, it is still limited to the small test stage, and there are still disadvantages such as complex extraction and separation processes, long production cycle, and low product yield.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Get the fresh camphor tree seeds and place them in a negative pressure cavitation mixing tank, add 1‰ potassium permanganate aqueous solution to immerse the seeds, and use a negative pressure of 0.08MPa to carry out cavitation mixing and wall breaking disinfection for 20 minutes. After taking it out, place it in a germination device, and germinate it in a greenhouse at a temperature of 20° C. for 10 days. Take 70Kg of philodendron seed buds and mix them with 300Kg of 70% ethanol extraction solvent, put them into a beater for homogenate extraction, use an 80-mesh filter to carry out suction filtration, and then mix the filter residue with an equal volume of 70% ethanol extraction solvent and place it in a negative pressure cavitation mixer. In the rotary solid-liquid extraction device, carry out negative pressure cavitation mixed rotary extraction for 20 minutes, then carry out suction filtration through an 80-mesh filter screen to obtain the filtrate, repeat the operatio...

Embodiment 2

[0024] Get the fresh camphora seed and place it in the negative pressure cavitation mixing tank, add 2‰ potassium permanganate aqueous solution to immerse the seeds, adopt negative pressure as 0.1MPa to carry out cavitation mixing tank wall breaking disinfection, the time is 30 minutes, After taking it out, put it in a germination device, and germinate it in a greenhouse at a temperature of 30° C. for 14 days. Get 70Kg of philodendron seed buds and mix them with 300Kg of 70% ethanol extractant and put them into a refiner for homogenate extraction, use a 200-mesh filter screen to carry out centrifugal filtration to obtain filtrate, and the filter residue is mixed with an equal volume of 70% ethanol extraction solvent and placed in a negative In the pressure-cavitation mixed-rotation solid-liquid extraction device, negative pressure cavitation mixed-rotation extraction was carried out for 40 minutes, and then passed through a 200-mesh filter to centrifugally filter to obtain the ...

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Abstract

The high-effective production method of 10-hydroxycamptothecin includes the following steps: making seed of camptotheca acuminate undergo the processes of negative pressure cavitation mixed-rotating wall-breaking, disinfecting, removing impurity, water culture and germination, mixing the seed sprout and extraction solvent, homogenization and solid-liquid extraction, filtration, negative pressure cavitation mixed-rotating solid-liquid extraction, filtration, concentration, negative pressure cavitation mixed-rotating liquid-liquid extraction, recrystallization or silicon gel column chromatography so as to obtain the 10-hydroxycamptothecin. Said invention raises its yield, can be substituted for traditional various extraction methods of drying material, pulverizing and heating process.

Description

Technical field: [0001] The invention relates to a method for producing 10-hydroxycamptothecin, in particular to a method for efficiently producing 10-hydroxycamptothecin by utilizing buds of camptothecin species. Background technique: [0002] At present, the anticancer effect of 10-hydroxycamptothecin is much better than that of similar anticancer drug camptothecin due to its small toxic and side effects and high curative effect. The content in plants is extremely low, only one in 100,000, and its market source is mainly obtained through chemical synthesis, which has high production costs and high market prices. [0003] Zu Yuangang's invention patent "Processing method for producing 10-hydroxycamptothecin and camptothecin raw materials", Patent Office of China Intellectual Property Office, application number: 02132410.7, the patent adopts the method of hydroponic germination to 10-hydroxycamptothecin Biotransformation of hydroxycamptothecin. The technical solution is: p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/00C07D491/22
Inventor 祖元刚史权祖柏实付玉杰唐中华张宝友
Owner NORTHEAST FORESTRY UNIVERSITY
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