Lactobacillus curvatus capable of promoting generation of short-chain fatty acid in intestinal tract and application of lactobacillus curvatus
A Lactobacillus campylobacter, product technology, applied in the fields of medicine and microbiology, to achieve good safety, reduce colon shortening, and reduce the effect of disease activity index
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Embodiment 1
[0086] Example 1: Screening, identification and culture of Lactobacillus Campylobacter CCFM1268
[0087] 1. Filter
[0088] The fermented kimchi water from Sichuan Province was used as the sample. After the sample was pretreated, it was stored in a refrigerator at -80 °C in about 30% glycerol. After taking out and thawing, the sample was mixed and 0.2 mL of the sample was added to 5 mL of LBS medium. After enrichment for 8 hours, the enriched solution was diluted with 0.9% normal saline, and the appropriate gradient dilution was coated on LBS solid medium, cultured at 37°C for 48 hours, and typical colonies were picked and streaked on the MRS plate for purification. A single colony was picked and transferred to liquid MRS liquid medium for enrichment, and stored in 30% glycerol to obtain strain CCFM1268.
[0089] 2. Identification
[0090] Extract the genome of the screened Lactobacillus campylobacter, amplify and sequence its 16S rDNA (completed by Shanghai Sangon Bioengine...
Embodiment 2
[0094] Example 2: Effects of Lactobacillus Campylobacter CCFM1268 on colon length, body weight change, disease activity index and colon tissue in mice with colitis
[0095] Thirty-two healthy female C57 mice aged 6-8 weeks were randomly divided into 4 groups, with 8 mice in each group. The 4 groups were: blank control group, modeling group, and intragastric administration of Lactobacillus campylobacter CCFM1268 and Lactobacillus sake QJSNT1L10 respectively. Bacterial suspension CCFM1268, QJSNT1L10 intervention group. Among them, each mouse in the blank group and the model group was intragastrically administered with normal saline at a dose of 0.2 mL once a day, and each mouse in the other groups was intragastrically administered 1×10 at a dose of 0.2 mL 9 CFU / mL bacterial suspension. During the experiment, the blank group was given normal drinking water, and the other groups were given 25g / L DSS drinking water. After the experiment, each mouse was anesthetized with isofluran...
Embodiment 3
[0107] Example 3: The effect of Lactobacillus campylobacter CCFM1268 on the expression of tight junction protein in the colon of mice with colitis
[0108] Thirty-two healthy female C57 mice aged 6-8 weeks were randomly divided into 4 groups, with 8 mice in each group. The 4 groups were: blank control group, modeling group, and intragastric administration of Lactobacillus campylobacter CCFM1268 and Lactobacillus sake QJSNT1L10 respectively. Bacterial suspension CCFM1268, QJSNT1L10 intervention group. Among them, each mouse in the blank group and the model group was intragastrically administered with normal saline at a dose of 0.2 mL once a day, and each mouse in the other groups was intragastrically administered 1×10 at a dose of 0.2 mL 9 CFU / mL bacterial suspension. During the experiment, the blank group was given normal drinking water, and the other groups were given 2.5% DSS drinking water. After the experiment, each mouse was anesthetized with isoflurane and sacrificed. ...
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