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Lactobacillus plantarum and application thereof in preparation of probiotic preparation for prawn culture

A technology of Lactobacillus plantarum and inhibitors, which is applied in the field of Lactobacillus plantarum and its application in the preparation of probiotic preparations for shrimp farming, which can solve the problems of difficult verification of active ingredients, complicated types and sources of lactic acid bacteria preparations, and unsatisfactory regulatory effects, etc. problems, achieve significant economic value, and increase growth and survival rates

Active Publication Date: 2022-08-09
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many types and sources of lactic acid bacteria preparations on the market, and it is difficult to prove the active ingredients. Its regulatory effect as the dominant bacteria in the intestinal tract of aquatic animals is not ideal

Method used

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  • Lactobacillus plantarum and application thereof in preparation of probiotic preparation for prawn culture
  • Lactobacillus plantarum and application thereof in preparation of probiotic preparation for prawn culture
  • Lactobacillus plantarum and application thereof in preparation of probiotic preparation for prawn culture

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Experimental program
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Effect test

specific Embodiment 1

[0023] 1. The separation and purification of the target strain

[0024] On the sterile super cleanliness, picking the alive pearl dragon gallbladder intestines with a sterilized toothpick is put into a sterilized 2 ml EP tube, adds a high -pressure sterilization 1.5 ml PBS buffer (pH = 7.0). S is placed at 28 ° C and 180 RPM shake in bed for 30 min; the cultivation solution after an appropriate amount of cultivation is diluted for gradient (10 -2 10 -3 10 -4 10 -5 ), Evenly coated in the MRS solid medium, and cultivate 37 ° C for 2-3 days; use the sterile inoculation ring to pick up the colonies, continue to be purified and cultivated on the MRS tablet twice, and then add 20%of the volume of the bacterial fluid volume. Soccinium glycerin is planted at -80 ° C.

[0025] 2. The bacteriostatic effect of the strain of the present invention

[0026] After activating the strain that is separated, it is cultivated at 28 ° C with MRS, and then the OD 600 And adjust its OD value to 0.6-0.8...

specific Embodiment 2

[0033] This experiment was conducted at the Qingjiang Breeding Base in the Institute of Marine Aquaculture, Zhejiang Province. A total of 10 400L (diameter 1m and a height of 0.8m) Vannabin Ecological Experimental Barrel was set up. Plants EP-M17 adding group. Put the shrimp seedlings uniformly, 200 tails of each experimental bucket (the average body length is 6.05 ± 0.45 cm, the average weight is 4.02 ± 0.54 g), and the breeding density is 500 tail / m tail / m / m. 3 After 7 days, the experiment was officially started. Robin activation and adding: Take the storage strain with the storage number CGMCC No. 24559. After overnight, then transferred to the fresh MRS broth with a ratio of 2%(V / V) to cultivate 24 h. At this time 9 -5 × 10 9 CFU / ML, and PH stabilizes between 4.0-4.1, obtains the EP-M17 bacterial liquid of Pottery. After collecting the bacteria from 500 mL fresh culture solution, washed the bacteria 3 times with sterile seawater, and then used the sterile seawater to heavy the...

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Abstract

The invention discloses lactobacillus plantarum and application thereof in preparing a probiotic preparation for prawn culture, and is characterized in that the strain is an Ep-M17 strain, is classified and named as lactobacillus plantarum, is preserved in China General Microbiological Culture Collection Center (CGMCC) on March 21, 2022, and has a preservation number of CGMCC No.24559; the lactobacillus plantarum is applied to preparation of a probiotic preparation for prawn culture, a vibrio parahaemolyticus inhibitor, a vibrio alginolyticus inhibitor, edwardsiella tarda, staphylococcus aureus, a pseudomonas plecoglossicida inhibitor, a plesiomonas shigelloides inhibitor, an aeromonas hydrophila inhibitor and an aeromonas veronii inhibitor. The compound has the advantages that the compound has an inhibition effect on edwardsiella tarda, staphylococcus aureus, vibrio parahaemolyticus, vibrio alginolyticus, pseudomonas plecoglossicida, plesiomonas shigelloides, aeromonas hydrophila and aeromonas veronii pathogens.

Description

Technical field [0001] The present invention involves a plant lactibilobe strain, especially the application of a plant lactobacillus and its application in preparation of poippymodogen in shrimp farmers. Background technique [0002] Aquatic cultivation occupies an important position in my country's fisheries. The intensive and high -density breeding has become the development trend of the aquaculture industry, but at the same time, it has caused frequent occurrence of aquatic animal diseases, which has greatly restricted the development of my country's aquaculture industry. Due to the rapid outbreak of aquatic animal diseases, strong infectious power, and many influencing factors, aquatic diseases are still mainly preventive, and the treatment of acute diseases can usually be controlled by antibiotics and chemicals. The long -term and large amount of antibiotics not only cause increased drug resistance to the aquatic animal, but also cause severe pollution to the environment, m...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K50/80A23K10/18C12R1/25
CPCC12N1/20A23K50/80A23K10/18C12R2001/25A23V2400/169Y02A40/81
Inventor 杜杨陈炯苗亮
Owner NINGBO UNIV
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