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Application of plasma-derived exosome lncRNA in preparation of drug-induced liver injury biomarker

A technology for biomarkers and liver injury, applied in the biological field, can solve the problems of complex detection methods, low sensitivity, and expensive reagents, and achieve the effects of reliable detection results, high sensitivity, and simple detection methods and reagents.

Active Publication Date: 2022-08-05
SHANGHAI INNOSTAR BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The technical problem to be solved by the present invention is to overcome the low sensitivity of drug-induced liver injury biomarkers in the prior art, the detection method is too complicated, the reagents are expensive, the liver injury needs to be evaluated in combination with other indicators, and the drug-induced liver injury cannot be predicted timely and accurately. Provides a use of exosome-derived long non-coding RNA (lncRNA) in the preparation of liver injury biomarkers and a kit containing it

Method used

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  • Application of plasma-derived exosome lncRNA in preparation of drug-induced liver injury biomarker
  • Application of plasma-derived exosome lncRNA in preparation of drug-induced liver injury biomarker
  • Application of plasma-derived exosome lncRNA in preparation of drug-induced liver injury biomarker

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Embodiment 1

[0039] Example 1 Searching for liver injury biomarkers

[0040] 1.1 Establishment of liver injury model

[0041] 1.1.1 Experimental reagents and instruments, experimental animals

[0042] (1) Positive drug: acetaminophen (batch number: D2114266, purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.);

[0043] (2) Solvent reference substance: 0.5% sodium carboxymethyl cellulose (0.5% CMC-Na);

[0044] ALT and AST detection kits are produced by Wako Pure Chemical Industries, Ltd.;

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Abstract

The invention discloses an application of a plasma-derived exosome lncRNA (long non-coding ribonucleic acid) in preparation of a drug-induced liver injury biomarker. The lncRNA comprises an lncRNA of which the NONCODE TRANSCRIPT ID (identity) is NONRATT018001.2. The invention further discloses a preparation method of the lncRNA. The lncRNA can be applied to preparation of the liver injury biomarker, especially used for detecting liver injury caused by exogenous compounds, the detection sensitivity is high, the detection method and reagent are simple, the lncRNA can be widely applied to detection of drug-induced liver injury, the detection result is reliable, and the method is suitable for popularization and application. The liver injury can be independently detected or jointly evaluated in combination with other indexes.

Description

technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to the use of plasma-derived exosome lncRNA in the preparation of drug-induced liver injury biomarkers. Background technique [0002] The liver is the most important metabolic organ in the body and one of the main target organs of exogenous toxins in the body. The susceptibility of the liver to chemical damage is determined by its special anatomical location and physiological and biochemical functions. Because the liver is anatomically close to the blood supply from the digestive tract, it is the primary target for toxic substances absorbed through the digestive tract to exert their toxic effects; the liver has the ability to concentrate and convert exogenous chemicals It plays an important role in the process of bile and its metabolites. Therefore, when the body is exposed to exogenous toxins, the liver is more often toxic than other organs. Therefore, there is an urgent ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 汤纳平杨紫轩常艳郑敏慧
Owner SHANGHAI INNOSTAR BIO TECH
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