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Application of circular RNA (Ribonucleic Acid) in preparation of breast cancer diagnosis product

A breast cancer and circular technology, applied in the field of biomedicine, can solve problems such as limited clinical value, low specificity and sensitivity, and heavy economic burden

Pending Publication Date: 2022-08-02
SHANDONG UNIV QILU HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Breast mammography is of great significance for the detection of early breast cancer, but it also has limitations such as missed diagnosis, delayed diagnosis, and heavy economic burden
However, conventional serum markers such as CEA and CA-153 are not specific and sensitive in the preoperative diagnosis of breast cancer, and their clinical value is limited.

Method used

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  • Application of circular RNA (Ribonucleic Acid) in preparation of breast cancer diagnosis product
  • Application of circular RNA (Ribonucleic Acid) in preparation of breast cancer diagnosis product
  • Application of circular RNA (Ribonucleic Acid) in preparation of breast cancer diagnosis product

Examples

Experimental program
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Effect test

Embodiment approach

[0039] The third embodiment of the present invention provides an application of a reagent for detecting circular RNA in the preparation of a preparation for screening breast cancer molecular markers, and the circular RNA is hsa_circ_0075796.

[0040] The fourth embodiment of the present invention provides a detection kit for breast cancer diagnosis, wherein the detection kit is equipped with a detection system for detecting hsa_circ_0075796 in breast cancer tissue.

[0041] In some examples of this embodiment, the detection system includes a reverse transcription RNA reaction system and a real-time quantitative polymerase chain reaction system.

[0042] In one or more embodiments, the reverse transcription RNA reaction system includes reverse transcription buffer solution, reverse transcriptase, nuclease-free pure water, reverse transcription primers, and the like.

[0043] In one or more embodiments, the real-time quantitative polymerase chain reaction system at least include...

Embodiment

[0048] (1) Clinical samples

[0049] From May 2017 to July 2021, 189 breast cancer patients were recruited at Qilu Hospital of Shandong University. All patients and controls were Han women.

[0050] Carcinoma and paracancerous tissues were collected from 189 breast cancer patients, and fresh tissue samples were immediately stored in liquid nitrogen until further analysis. All breast cancer and adjacent tissues were obtained during surgery. All participants obtained written informed consent. The study in this example was approved by the Ethics Committee of Qilu Hospital of Shandong University.

[0051] (2) RNA extraction

[0052] 1) Take out 30-50 mg of tissue samples stored in liquid nitrogen and quickly transfer them to an EP tube, add 700 μl of RNA-easyIsolation Reagent, and use tissue scissors to fully shred the tissue until it becomes a paste (no visible particles). . Add 280ml of RNase-free ddH to the above lysate 2 O, invert up and down to mix, let stand at room t...

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Abstract

The invention belongs to the technical field of biological medicines, and relates to application of circular RNA (Ribonucleic Acid) in preparation of a breast cancer diagnosis product. The breast cancer diagnosis product can be used for detecting the expression of circular RNA in a tissue sample to be detected, and the circular RNA is hsacirc0075796. Compared with a para-carcinoma control group, hsacirc0075796 is found to be obviously down-regulated in breast cancer tissues and is related to breast cancer lymph node metastasis, HER2 expression, relatively large tumor diameter, relatively high Ki-67 expression level, relatively high histological grading, more invasive molecular typing and later clinical staging, so that definite diagnosis and malignant degree detection of breast cancer are realized.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to the application of a circular RNA in the preparation of a product for diagnosing breast cancer. Background technique [0002] The information disclosed in this Background section is only for enhancement of understanding of the general background of the invention and should not necessarily be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art. [0003] Breast cancer is one of the tumors with the highest incidence in women, and it is also one of the main reasons why women die of cancer. At the same time, breast cancer patients also face a huge risk of metastasis and recurrence. So early detection and diagnosis of breast cancer is very important. Mammography is of great significance for the detection of early breast cancer, but it also has limitations such as missed diagnosis, dela...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12Q1/6886C12N15/11C12Q1/6851A61K45/00A61P35/00
CPCC12N15/113C12Q1/6886C12Q1/6851A61K45/00A61P35/00C12N2310/532C12Q2600/118C12Q2600/158C12Q2600/166C12Q2600/136C12Q2600/106C12Q2525/307C12Q2561/113C12Q2531/113C12Q2521/107C12Q2527/125Y02A50/30
Inventor 张凯王亚文徐瑶杨斌陈旭
Owner SHANDONG UNIV QILU HOSPITAL
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