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PGPR ecological restoration agent and preparation method thereof

A technology of ecological restoration and bacterial species, applied in biochemical equipment and methods, microorganism-based methods, chemical instruments and methods, etc., can solve problems such as affecting the growth of plankton fish in water bodies, slow restoration process, and residual chemical components. , to achieve good water body repair function, increase the content, and prevent the outbreak of algal blooms

Pending Publication Date: 2022-07-29
江西调水人生态环境工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of PGPR ecological restorative agent and preparation method, to solve the problem in the prior art, water body restoration mostly adopts chemical repair agent, the restoration process of this restoration agent is slow, and restoration effect is poor, and the chemical restoration agent in the restoration agent Components remain in the water body, affecting the growth of plankton and fish in the water body

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A PGPR ecological restoration agent, in terms of mass percentage, the components and contents are as follows:

[0037] 5% of bacillus, 30% of lactic acid bacteria, 30% of photosynthetic bacteria, 34% of yeast, and 1% of vitamin.

[0038] A preparation method of a PGPR ecological restoration agent, comprising the following steps:

[0039] S1, culture Bacillus:

[0040] Bacillus is cultured in peptone nutrient medium, the culture temperature is 25~30℃, the pH value is 7.0~7.5, and oxygen is introduced to make the dissolved oxygen in the culture medium not less than 2mg / L, and the culture time is 24~48h ;

[0041] S2, culture lactic acid bacteria:

[0042] The lactic acid bacteria are cultured in a molasses medium, the content of the molasses is 5-10%, and the culture temperature is 35-37 DEG C, and the anaerobic culture is carried out for 5-10 days;

[0043] S3, culturing photosynthetic bacteria:

[0044] The photosynthetic bacteria are cultured in an inorganic salt ...

Embodiment 2

[0057] A PGPR ecological restoration agent, in terms of mass percentage, the components and contents are as follows:

[0058] 10% of bacillus, 25% of lactic acid bacteria, 20% of photosynthetic bacteria, 40% of yeast, and 5% of vitamins.

[0059] A preparation method of a PGPR ecological restoration agent, comprising the following steps:

[0060] S1, culture Bacillus:

[0061] Bacillus is cultured in peptone nutrient medium, the culture temperature is 25~30℃, the pH value is 7.0~7.5, and oxygen is introduced to make the dissolved oxygen in the culture medium not less than 2mg / L, and the culture time is 24~48h ;

[0062] S2, culture lactic acid bacteria:

[0063] The lactic acid bacteria are cultured in a molasses medium, the content of the molasses is 5-10%, and the culture temperature is 35-37 DEG C, and the anaerobic culture is carried out for 5-10 days;

[0064] S3, culturing photosynthetic bacteria:

[0065] The photosynthetic bacteria are cultured in an inorganic sal...

Embodiment 3

[0078] A PGPR ecological restoration agent, in terms of mass percentage, the components and contents are as follows:

[0079] 5% of bacillus, 30% of lactic acid bacteria, 30% of photosynthetic bacteria, 30% of yeast, and 5% of vitamins.

[0080] A preparation method of a PGPR ecological restoration agent, comprising the following steps:

[0081] S1, culture Bacillus:

[0082] Bacillus is cultured in peptone nutrient medium, the culture temperature is 25~30℃, the pH value is 7.0~7.5, and oxygen is introduced to make the dissolved oxygen in the culture medium not less than 2mg / L, and the culture time is 24~48h ;

[0083] S2, culture lactic acid bacteria:

[0084] The lactic acid bacteria are cultured in a molasses medium, the content of the molasses is 5-10%, and the culture temperature is 35-37 DEG C, and the anaerobic culture is carried out for 5-10 days;

[0085] S3, culturing photosynthetic bacteria:

[0086] The photosynthetic bacteria are cultured in an inorganic salt...

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Abstract

The invention is applicable to the technical field of water body restoration, and provides a PGPR ecological restoration agent and a preparation method thereof, and the preparation method comprises the following steps: S1, culturing bacillus; s2, culturing lactic acid bacteria; s3, culturing the photosynthetic bacteria; s4, culturing saccharomycetes; s5, centrifuging and separating; s6, strain mixing; s7, adding vitamins, and stirring; the product obtained by the PGPR ecological restoration agent and the preparation method provided by the invention has a good water body restoration function, can purify water, is ecological and environment-friendly, and does not cause secondary environmental pollution, and when the product obtained by the PGPR ecological restoration agent and the preparation method provided by the invention is applied, the product has a good application prospect. Vitamin in the carrier is an indispensable growth factor for growth and reproduction of microorganisms, and endogenous indigenous microorganisms and exogenously added microorganisms can be quickly activated when the vitamin is put into a water body; the microorganisms and activated indigenous microorganisms in the product can provide food for zooplankton in the water body.

Description

technical field [0001] The invention relates to the technical field of water body restoration, in particular to a PGPR ecological restoration agent and a preparation method. Background technique [0002] In recent years, with the rapid development of economy and industry, it has caused great pollution to water bodies. Water pollution has seriously affected economic development and people's lives. Therefore, water body restoration is imminent. [0003] Most of the existing water body restoration uses chemical restoration agents. The restoration process of this restoration agent is slow and the restoration effect is poor. Moreover, the chemical components in the restoration agent remain in the water body, which affects the growth of plankton and fish in the water body. SUMMARY OF THE INVENTION [0004] The purpose of the present invention is to provide a PGPR ecological restoration agent and a preparation method, so as to solve the problem that in the prior art, chemical res...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/38C12N11/14C02F3/34C02F3/10C12R1/07C12R1/225C12R1/01C12R1/645
CPCC12N1/20C12N1/16C12N1/38C12N11/14C02F3/34C02F3/347C02F3/106C02F3/107C02F2305/06
Inventor 陶妹英曾庆辉
Owner 江西调水人生态环境工程有限公司
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