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Preparation method of freeze-dried powder containing placenta amniotic stem cell cytokine extracting solution

A technology of cytokines and extracts, which is applied in the field of preparation of freeze-dried powder containing cytokine extracts from placental amniotic membrane stem cells, can solve the problems of short storage time and increased cost investment, and achieve improved anti-corrosion performance, reduced investment, and improved preservation. the effect of time

Inactive Publication Date: 2022-07-29
上海南滨江细胞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The prior art has the following disadvantages: the existing freeze-dried powder has a short storage time, which will increase the cost of input

Method used

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  • Preparation method of freeze-dried powder containing placenta amniotic stem cell cytokine extracting solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation method of the freeze-dried powder containing the placental amniotic membrane stem cell cytokine extract provided by the present invention includes the following specific steps:

[0036] S1, the first material is put into a mixer for stirring and heating, the stirring temperature is 55°C, the time is 25min, and the mixture is filtered to obtain a mixture A, which is stored in an environment of 4°C for later use, wherein the first material is It is composed of extract A, whey protein concentrate powder and stachyose, the first material (by weight) is: extract A 10g, whey protein concentrate powder 12g, stachyose 8g;

[0037] The preparation steps of extract A are as follows:

[0038] Step 1: put the camellia seed husk powder, pomegranate oil seed husk powder and water into a mixer for stirring, the stirring temperature is 50°C, and the time is 20min, thereby obtaining a mixture;

[0039] Step 2: filter the mixture to obtain extract A;

[0040] S2, the se...

Embodiment 2

[0049] The preparation method of the freeze-dried powder containing the placental amniotic membrane stem cell cytokine extract provided by the present invention includes the following specific steps:

[0050] S1, put the first material into a mixer for stirring and heating, the stirring temperature is 57.5°C, the time is 27.5min, and the mixture is filtered to obtain a mixture A, which is preserved in an environment of 5°C for later use, wherein the first The material is composed of extract A, whey protein concentrate powder and stachyose, and the first material (by weight) is: extract A 12.5g, whey protein concentrate powder 14g, and stachyose 9g;

[0051] The preparation steps of extract A are as follows:

[0052] Step 1: put the camellia seed husk powder, pomegranate oil seed husk powder and water into a mixer for stirring, the stirring temperature is 55°C, and the time is 25min, thereby obtaining a mixture;

[0053] Step 2: filter the mixture to obtain extract A;

[0054] ...

Embodiment 3

[0063] The preparation method of the freeze-dried powder containing the placental amniotic membrane stem cell cytokine extract provided by the present invention includes the following specific steps:

[0064] S1, put the first material into a mixer for stirring and heating, the stirring temperature is 60°C, the time is 30min, and filter to obtain mixture A, which is stored in an environment of 6°C for later use, wherein the first material is It is composed of extract A, whey protein concentrate powder and stachyose, and the first material (by weight) is: extract A 15g, whey protein concentrate powder 16g, stachyose 10g;

[0065] The preparation steps of extract A are as follows:

[0066] Step 1: put the camellia seed husk powder, pomegranate oil seed husk powder and water into a mixer for stirring, the stirring temperature is 60°C, and the time is 30min, thereby obtaining a mixture;

[0067] Step 2: filter the mixture to obtain extract A;

[0068] S2, the second material is ...

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Abstract

The invention discloses a preparation method of freeze-dried powder containing a placenta amniotic stem cell cytokine extracting solution, and relates to the technical field of freeze-dried powder, the technical scheme is that the preparation method comprises the following specific steps: S1, putting a first material into a stirrer for stirring and heating, and filtering to obtain a mixture A, the first material is prepared from an extracting solution A, whey protein concentrate powder and stachyose, and the second material is prepared from a second material, a third material and a fourth material; s2, putting the second material into a stirrer for stirring and heating, filtering to obtain a mixture B, and storing the mixture B for later use in an environment of 4-6 DEG C. The preparation method of the freeze-dried powder containing the placenta amniotic membrane stem cell cytokine extracting solution has the beneficial effects that the preservative has the effects of improving the corrosion resistance of the freeze-dried powder; therefore, the preservation time of the freeze-dried powder is prolonged, and the cost investment is reduced.

Description

technical field [0001] The invention relates to the technical field of freeze-dried powder, in particular to a method for preparing a freeze-dried powder containing a placental amniotic membrane stem cell cytokine extract. Background technique [0002] Freeze-dried powder is a sterile powder injection obtained by freezing the medicinal liquid into a solid state in a sterile environment, and vacuuming to sublime and dry the water. Freeze-dried powder is obtained by freezing the moisture in the liquid in advance by the vacuum freeze-drying method of a freeze dryer, and then subliming the frozen moisture in the liquid in a vacuum aseptic environment, thereby obtaining freeze-drying. In short, the water in the medicinal solution is drained in a low temperature environment to retain its original medicinal effect. [0003] The prior art has the following deficiencies: the storage time of the existing freeze-dried powder is short, thereby increasing the cost of investment. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/50A61K9/19A61P31/00
CPCA61K35/50A61K9/19A61P31/00
Inventor 占震锋李庆静
Owner 上海南滨江细胞生物科技有限公司
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