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Application of Penicillium Polish extract in preparation of herbicide

A technology of extracts and herbicides, applied in the field of biological control, can solve problems such as environmental pollution, herbicide dosage, crop injury risk, and increase the cost of weed control, achieve high weeding efficiency, wide application value, and solve weeds The effect of resistance problems

Active Publication Date: 2022-07-29
SUZHOU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, the long-term, single, and extensive use of herbicides has led to an increase in resistant weed individuals and populations year by year, reducing the control effect of herbicides and increasing the cost of weed control; at the same time, the increase in herbicide dosage is also easy Risk of crop injury and environmental pollution

Method used

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  • Application of Penicillium Polish extract in preparation of herbicide
  • Application of Penicillium Polish extract in preparation of herbicide
  • Application of Penicillium Polish extract in preparation of herbicide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Preparation of Penicillium Polonia Extract

[0029] Respectively activate the strain of Penicillium polandum No. 1 (preservation unit: China Agricultural Microorganism Culture Collection and Management Center, preservation number: ACCC 31573) and / or Penicillium polandicus strain No. 2 (preservation unit: China Center for Type Culture Collection, preservation number: No. M2010086), use PDB medium for liquid fermentation for 7 days, filter 2L of the fermentation broth with gauze, extract the obtained liquid with ethyl acetate, and perform rotary evaporation of the obtained organic phase to obtain crude extract I. The bacterial cells obtained were filtered with gauze, soaked in methanol, sonicated for 30 min, and then filtered. The obtained liquid was subjected to rotary evaporation to obtain crude extract II. The crude extracts obtained twice were mixed and prepared into an extract of 100 mg / mL with methanol. .

[0030] The Penicillium Poloniae extract is elut...

Embodiment 2

[0041] Embodiment 2, activity test

[0042] 1) Test the inhibitory activity of the fermented liquid extract of Penicillium polish 1 strain on the germination of Chinese cabbage and crabgrass

[0043] Experimental method: filter 2L of the 7-day fermentation broth with gauze, extract the obtained liquid with ethyl acetate, and perform rotary evaporation on the obtained supernatant to obtain crude extract I. The bacterial cells obtained were filtered with gauze, soaked in methanol, sonicated for 30 min, and then filtered. The obtained liquid was subjected to rotary evaporation to obtain crude extract II. The crude extracts obtained twice were mixed and prepared into an extract of 100 mg / mL with methanol. mother liquor.

[0044]Seed surface disinfection: the seeds (smooth surface, flat seeds, no cracking, complete appearance) are first rinsed with sterile water, then soaked with 70% ethanol for 2 minutes, then soaked with 2% sodium hypochlorite for 2 minutes, and finally with ste...

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Abstract

The invention discloses application of a Penicillium Polish extract in preparation of herbicides. Penicillium Polish is a bacterial strain preserved in the China Agricultural Microbiological Culture Collection Center, and the preservation number is ACCC 31573. The extract is identified to contain compounds shown as a formula I, a formula II and a formula III, the formula II has good herbicidal activity and can be used for weeds of dicotyledonous plants and monocotyledonous plants, the research further finds that the compound component containing the formula II has higher herbicidal efficiency, the problem of weed resistance in the prior art can be solved, and the compound component has broad application prospects. Therefore, the herbicide has wide application value.

Description

technical field [0001] The invention relates to the field of biological control, in particular to an extract of Penicillium Poloniae, and also relates to the application of an extract of Penicillium Poloniae in the preparation of herbicides. Background technique [0002] Weeds will seriously threaten the yield of crops and cause huge economic losses to agricultural production. Compared with artificial weeding, herbicides have the advantages of economy, rapidity and high efficiency, which provide an important guarantee for high and stable agricultural yields. However, in recent years, the long-term, single, and extensive use of herbicides has led to the increase of resistant weed individuals and populations year by year, reducing the control effect of herbicides and increasing the cost of weed control. Cause crop phytotoxicity risk and environmental pollution. At present, the problem of weed resistance has become one of the important problems threatening the production of f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/08A01N43/90A01P13/00
CPCA01N43/08A01N43/90Y02A50/30
Inventor 张浩白净何姿良徐玉泉纪莉莲扶教龙
Owner SUZHOU UNIV OF SCI & TECH
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