Verification equipment and method of DNA biosensor for water pollution detection

Abstract

The invention discloses a verification device and method of a DNA biosensor for water pollution detection, the verification device comprises a fluorescence spectrometer, a solution tank, a controller and a reference signal memory, the fluorescence spectrometer and the DNA biosensor are both connected with the controller, the DNA biosensor is arranged in the solution tank, and the reference signal memory is connected with the controller. The fluorescence spectrometer is used for collecting fluorescence signals emitted after detection of the DNA biosensor, the reference signal memory is connected to the controller, and stable fluorescence intensities corresponding to different pathogenic microorganisms in the water pollution solution are stored in the reference signal memory. According to the invention, the working state of the DNA biosensor is verified by comparing the light intensity of the detection signal with the light intensity of the reference signal, so that the test accuracy is ensured.

Description

technical field [0001] The invention relates to the technical field of water pollution detection equipment, in particular to a verification equipment and method of a DNA biosensor for water pollution detection. Background technique [0002] DNA biosensor is an analysis device made of a combination of specific sequence probes and signal converters. It has the characteristics of real-time and easy operation in gene hybridization diagnosis. Therefore, it is widely used in the fields of biology, medicine, environmental monitoring and food. They have a wide range of applications, and more and more applications need to verify the sensitivity of DNA biosensors, but there is currently no effective test method. Since the sensitivity of the DNA biosensor varies with each sticking process, and sometimes the difference is large, it is necessary to test and verify the working state of the DNA biosensor. However, the existing test and verification methods are difficult to reasonably evalu...

AI Technical Summary

Problems solved by technology

[0002] DNA biosensor is an analytical device made by combining specific sequence probes and signal converters. It has real-time and easy-to-operate characteristics in gene hybridization diagnosis, so it is widely used in the fields of biology, medicine, environmental monitoring and food. There are a wide range of applications, and more and more applications need to verify the sensitivity of DNA biosensors, but there is no effective test method at present

Since the sensitivity of the DNA biosensor varies with each pasting process, and sometimes the difference is relatively large, it is necessary to test and verify the working status of the DNA biosensor. However, the existing test and verification methods are difficult to reasonably evaluate DNA. The required reproducibility or practical implementation requirements of biosensors may lead to inappropriate assessment of the function of DNA biosensors by operators or technicians

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