Method for breeding beet male sterile line and maintainer line by using excellent pollination line

Abstract

The invention provides a method for breeding a beet male sterile line and a maintainer line by using an excellent pollination line, which comprises the following steps: hybridizing an excellent single-grain beet maintainer line with a beet pollination line to obtain O * P seeds; selecting a single plant from the O * P filial generation as a male parent, performing plant-to-plant test cross on the male parent and a beet male sterility test line, and separately harvesting seeds to obtain CMS-n * (O * P)-n and (O * P)-n seeds; sowing the CMS-n * (O * P)-n seeds to cultivate mother roots, and investigating fertility during flowering; sowing seeds harvested from a male parent single plant of which the offspring is completely sterile in CMS plant-to-plant test cross, cultivating a mother root, independently isolating, opening and pollinating, and harvesting the seeds in a mixed manner; obtaining a beet maintainer line; a CMS test line used for test cross is a male sterile line. By adopting the method, excellent characters in the pollination line can be introduced into the O-type line, the O-type line is improved within a short time, and the CMS and the O-type line are rapidly bred.

Description

technical field [0001] The invention belongs to the technical field of sugar beet breeding, and in particular relates to a method for selecting a male sterile line and a maintainer line of sugar beet by utilizing an excellent pollination line. Background technique [0002] At present, the commercial varieties used in sugar beet production at home and abroad are single-seed male sterile three-cross hybrids. The most critical link in the production of commercial sugar beet hybrids is the selection of single-seed CMS (male sterile line) and O-type line (maintenance line). nurture. Since sugar beet is a biennial crop under normal circumstances, root vegetative growth is carried out in the first year, and the tuberous roots after low temperature vernalization are planted into the field in the second year for reproductive growth and flowering and seeding. At present, the conventional method of plant-to-plant pairwise hybridization and backcrossing is widely used in China to selec...

AI Technical Summary

Problems solved by technology

At present, the conventional method of plant-to-plant pair hybridization and then backcrossing is generally used in China to breed CMS and O-type lines of sugar beet. It takes at least 7 to 10 years for successful breeding, and with the increase of breeding generations , its breeding workload is growing in a pyramid style, and its selection work is labor-intensive and time-consuming

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