Distant hybrid breeding method of red-spotted grouper and saddle-banded grouper
A kind of technology of red spot grouper and saddle grouper
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[0034] The invention relates to a crossbreeding method of a red-spotted grouper female and a saddle-banded grouper male, which comprises parent selection before temperature control, parent cultivation, parent nutrition strengthening, parent artificial induction, artificial insemination and hatching.
[0035] 1. Parental selection before temperature control
[0036] This experiment was carried out from January to February 2015. The female red grouper was selected from 3-5 years old, weighing 0.5-1kg, normal body shape, strong vitality, and no nerve necrosis virus-carrying red grouper. The male of the saddle grouper is selected to be above 6 years old, with normal body shape, strong vitality, and no nerve necrosis virus-carrying grouper. Virus detection is to take blood through the tail artery, which is conducive to the detection of nerve necrosis virus by fluorescent quantitative PCR. The grouper with negative test results is selected as broodstock and raised separately.
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