Sorting method of fluorescence coding microspheres

A technology of fluorescent coding and microspheres, which is applied in the field of sorting fluorescent coding microspheres, can solve problems such as insufficient flexibility and complicated manufacturing process, and achieve the effects of simplifying the process, easy operation and improving efficiency

Pending Publication Date: 2022-07-01
COLORTECH SUZHOU BIOTECHNOLOGY CO LTD
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Problems solved by technology

[0006] The general metal dielectrophoretic force design, using micro-electromechanical process, first deposits a metal layer on the glass surface, then defines the shape of the electrode by photoresist, after etching and developing, it can form a metal pattern (conductive layer) at the micron scale, and then in The electrophoretic force is generated in the channel (microfluidic pipeline), but the pattern of the metal electrode is fixed, and the shape of the electric field is also fixed, so the flexibility is not enough, and the manufacturing process is also complicated, requiring special manufacturing technology and training

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  • Sorting method of fluorescence coding microspheres
  • Sorting method of fluorescence coding microspheres
  • Sorting method of fluorescence coding microspheres

Examples

Experimental program
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Effect test

Embodiment 1

[0071] see figure 1 and figure 2 , the photoelectric positioning and coding microsphere detection device can include a photoelectric chip, a signal generator (specific electrical signal control system), a group of optical beam reduction lens group (reduced by 20 times), light pattern control projection system (both to achieve specific optical system), a camera (CCD) (specific image processing system), and a micropump that controls liquid injection (specific fluid control system).

[0072] The photoelectric chip uses ITO conductive glass as the transparent electrode, including the upper layer ITO electrode and the lower layer ITO electrode, the ITO electrodes are respectively arranged on the glass substrate, the lower layer ITO is coated with a layer of photoconductive material, and the distance between the upper layer ITO electrode and the lower layer ITO electrode is maintained at 100 distance in microns. The area of ​​the ITO electrode is 1 cm × 1 cm. The upper layer ITO...

Embodiment 2

[0076] A real-time fluorescent coding microsphere sorting method, the light pattern used is such as Figure 8 As shown, the working area of ​​the photoelectric chip is divided into three parts, the first part is the particle gathering area and the real-time detection area, the second part is the light pattern conversion area, and the third part is the sorting and separation area. The details are as Figure 5 , Image 6 , Figure 7 shown.

[0077]When three kinds of encoded microspheres (for example, numbered (1), (2), (3) respectively) are mixed in the sample, they will be grabbed for different molecules. The way of automated micropump enters the optoelectronic chip. Since the encoded microspheres are scattered and random, they need to be arranged in a straight line and detected in real time.

[0078] like Figure 5 As shown in the figure, where the light pattern illuminates, a virtual electrode will be formed. When the particle encounters the light pattern, it will be pu...

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Abstract

The invention discloses a sorting method of fluorescent coded microspheres, which comprises the following steps: mixing different coded microspheres in a to-be-detected sample, and respectively grabbing corresponding different target molecules to obtain to-be-detected liquid; controlling the to-be-detected liquid to sequentially enter an accumulation area, a real-time detection area, a light pattern conversion area and a sorting separation area of the photoelectric chip through a fluid control system, so as to realize sorting of the fluorescent coded microspheres and corresponding to-be-detected molecules. According to the invention, the direction of dielectrophoretic force is changed by using the light guide material in combination with the light pattern design and changing the electric field distribution on the plane, so that the stress of the coding microspheres is changed, the purposes of positioning, grabbing, releasing and moving the coding microspheres are achieved, and the sorting and immunodetection efficiency of the coding microspheres is improved.

Description

technical field [0001] The invention belongs to the field of biological detection, in particular to a sorting method for fluorescently encoded microspheres. Background technique [0002] The detection of many cancers is closely related to the concentration of cytokines, and sometimes a single cancer cell can cause the release of multiple cytokines. In clinical practice, the amount and concentration of interleukins detected in the serum of patients can be used as indicators for early cancer diagnosis and after treatment. For example, the measurement of cytokines such as IL-1, IL-2, IL-6, IL-8, IL-10, IL-15, and IL-17 can be used for clinical detection of liver cancer, lung cancer, pancreatic cancer and ovarian cancer. For lung, breast, and ovarian cancers, most treatment times are less than 1 year from disease detection to ineffective treatment. If multiple cytokines can be detected in serum, the accuracy of cancer diagnosis and the survival rate of cancer patients can be i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N33/543
CPCG01N33/533G01N33/54313
Inventor 不公告发明人
Owner COLORTECH SUZHOU BIOTECHNOLOGY CO LTD
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