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Biological detection method and device based on mobile phone

A technology for biological detection and mobile phones, applied in the field of biological detection methods and devices based on mobile phones, can solve problems such as volume and cost constraints, and achieve the effects of extended functions, wide multiple quantitative biological detection, and wide dynamic range.

Pending Publication Date: 2022-06-28
FACON CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This challenge is especially obvious for point-of-care readers, because their size and cost are strictly limited, so the measurement dynamic range of the usual point-of-care readouts can only be about 2 orders of magnitude

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  • Biological detection method and device based on mobile phone
  • Biological detection method and device based on mobile phone
  • Biological detection method and device based on mobile phone

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Embodiment Construction

[0042] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0043] The mobile phone has powerful functions. The present invention cleverly utilizes the photoelectric principle of the existing mobile phone color photography and its components, and adds appropriate components to it at a minimum, so as to transform it into a convenient biological detection with wide dynamic range and multiple quantitative features. platform.

[0044] The purpose of the present invention is to provide a biological...

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Abstract

The invention relates to a biological detection method and device based on a mobile phone. The method comprises the following steps: selecting N quantum dots with specific wavelengths to respectively mark N indexes to be detected in a sample; n is 1, 2 or 3; exciting the to-be-detected index-quantum dot compound by utilizing exciting light; shooting the excited compound for multiple times by using different sensitivities and different exposure time combinations to obtain a 16-bit depth RAW format first original image file; respectively photographing the unmarked quantum dots to obtain a 16-bit deep RAW format second original image file; calculating a proportionality coefficient matrix of fluorescence intensity of each quantum dot received by each channel in the second original image file; calculating the fluorescence intensity of each to-be-detected index-quantum dot compound of the first original image file; and obtaining the concentration of each to-be-detected index according to the fluorescence intensity to complete biological detection. According to the invention, the functions of the mobile phone are greatly expanded, and a wide dynamic range and multiple quantitative detection can be realized.

Description

technical field [0001] The present invention relates to the technical field of biological detection, in particular to a mobile phone-based biological detection method and device. Background technique [0002] POCT (point-of-care testing, point-of-care testing) technology has developed rapidly, and has been able to qualitatively detect and quantitatively measure a number of biochemical indicators to help evaluate people's health. But so far, all quantitative POCT techniques have required a "reader" that quantifies the response signal, such as fluorescence. The commonly used "reader" includes at least two parts: one is the excitation part, that is, the signal substance in the detected object is excited (irradiated) with a light beam of a certain wavelength; the other is the receiving part, which receives the signal substance when it is excited (irradiated). The signal is then emitted and quantified. A fluorescence reader is a typical example of such an instrument. Due to th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N33/558
CPCG01N21/6428G01N21/64G01N21/6456G01N33/558G01N2021/6423G01N2021/6439G01N2021/6471G01N2021/6478G01N2021/6421G01N2201/129G01N2201/0221G01N2201/062G01N2021/8488
Inventor 王锋颜文卿詹祥
Owner FACON CO LTD
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