Prostate cancer metabonomics marker and screening method thereof

A technology of metabolomics and prostate cancer, applied in scientific instruments, biological testing, biomaterial analysis, etc., can solve the problems of incomplete sedimentation and low sedimentation efficiency, and achieve the effects of avoiding accuracy, improving efficiency, and rapid adsorption

Pending Publication Date: 2022-06-24
王海峰 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide prostate cancer metabolomics markers and screening methods thereof, which are obtained by purifying the blood with a sedimentation agent after removing high-abundance proteins, and then performing fluorescent staining and two-dimensional gel electrophoresis. The impurities in the solution are adsorbed to form insoluble precipitates, which solves the problem of low settling efficiency and incomplete settling of traditional settling agents

Method used

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Embodiment 1

[0024] A metabolomic marker of prostate cancer is obtained by removing high-abundance proteins from blood, purifying with a sedimentation agent, and then performing fluorescent staining and two-dimensional gel electrophoresis to obtain a metabolomic marker of prostate cancer.

[0025] The prostate cancer metabolomic markers were screened by the following steps:

[0026] Step S1: Shake the blue medium well, centrifuge for 5 s at a speed of 10000 r / min, remove the supernatant, add the substrate to the equilibration solution, continue centrifugation for 5 s, remove the supernatant, and add the substrate to the equilibration solution , continue centrifugation for 20s, remove the supernatant, mix the substrate and plasma evenly, incubate for 5min at a temperature of 20°C, centrifuge for 60s at a speed of 10000r / min, and continue to incubate the supernatant for 5min , centrifuge again, take the supernatant to keep warm to obtain the first sample, add the substrate to the equilibrium...

Embodiment 2

[0035] A metabolomic marker of prostate cancer is obtained by removing high-abundance proteins from blood, purifying with a sedimentation agent, and then performing fluorescent staining and two-dimensional gel electrophoresis to obtain a metabolomic marker of prostate cancer.

[0036] The prostate cancer metabolomic markers were screened by the following steps:

[0037] Step S1: Shake the blue medium well, centrifuge for 8s at a speed of 10000r / min, remove the supernatant, add the substrate to the equilibration solution, continue centrifugation for 8s, remove the supernatant, and add the substrate to the equilibration solution , continue centrifugation for 30s, remove the supernatant, mix the substrate and plasma evenly, incubate for 8min at a temperature of 23°C, centrifuge for 60s at a speed of 11000r / min, and continue to incubate the supernatant for 8min , centrifuge again, take the supernatant to keep warm to obtain the first sample, add the substrate to the equilibrium so...

Embodiment 3

[0046] A metabolomic marker of prostate cancer is obtained by removing high-abundance proteins from blood, purifying with a sedimentation agent, and then performing fluorescent staining and two-dimensional gel electrophoresis to obtain a metabolomic marker of prostate cancer.

[0047] The prostate cancer metabolomic markers were screened by the following steps:

[0048] Step S1: Shake the blue medium well, centrifuge for 10s at a speed of 10000r / min, remove the supernatant, add the substrate to the equilibration solution, continue centrifugation for 10s, remove the supernatant, and add the substrate to the equilibration solution , continue centrifugation for 40s, remove the supernatant, mix the substrate and plasma evenly, incubate for 10min at a temperature of 25°C, centrifuge for 60s at a speed of 12000r / min, and continue to incubate the supernatant for 10min , centrifuge again, take the supernatant to keep warm to obtain the first sample, add the substrate to the equilibriu...

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Abstract

The invention discloses a prostate cancer metabonomics marker and a screening method thereof, blood is subjected to abundance protein removal treatment and then purified by a settling agent, then fluorescent staining and two-way gel electrophoresis are performed to obtain the prostate cancer metabonomics marker, and carboxyl on carboxymethyl chitosan and amino on the surface of silicon dioxide are subjected to dehydration condensation to obtain the prostate cancer metabonomics marker. The preparation method comprises the following steps: preparing modified chitosan, carrying out hydrolytic polymerization on aluminum chloride and ferric chloride, carrying out loading by using the modified chitosan as a carrier to prepare composite chitosan, blending the composite chitosan with starch, and grafting with an acrylamide copolymer to prepare the settling agent. Compared with a traditional settling agent, the settling agent can more quickly adsorb impurities in plasma to form insoluble precipitates, so that the plasma protein purification efficiency is improved, and the influence of the impurities on the screening accuracy of the metabonomics markers of the adenocarcinoma is avoided.

Description

technical field [0001] The invention relates to the technical field of metabolomic marker screening, in particular to a metabolomic marker of prostate cancer and a screening method thereof. Background technique [0002] Prostate cancer is a common malignant tumor among elderly men. According to the latest statistics, there were nearly 1.3 million new cases and 359,000 deaths worldwide in 2018, and its incidence accounted for 13.5% of male malignant tumors, second only to lung cancer. It ranks second, and the mortality rate accounts for 6.7% of male malignant tumors, ranking fifth. Metabolomics is a relatively new discipline in systems biology, a scientific field that studies the metabolites produced by the biological metabolism of cells, tissues or organs. Since metabolites are relatively stable in body fluids, endogenous metabolic profiling has been more used in the early diagnosis of tumors in recent years, while prostate cancer is diagnosed by metabolomic markers in urin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N1/28C08F251/00C08F220/56
CPCG01N33/68G01N1/28C08F251/00G01N2800/7028G01N2550/00C08F220/56
Inventor 王海峰陈志超黄应龙付什栾婷李义
Owner 王海峰
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