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Piece preparation method for simultaneously observing and counting lower glandular hair, non-glandular hair and stomata of folium artemisiae argyi

A technique for the glandular and glandular hairs of Artemisia argyi, which is applied in the field of plant microscopic identification, can solve the problem that the distribution of glandular hairs, non-glandular hairs and stomata on Artemisia argyi leaves cannot be observed at the same time, and achieves a low workload, high success rate, and clear observation. Effect

Pending Publication Date: 2022-06-10
HENAN UNIV OF CHINESE MEDICINE
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Problems solved by technology

[0004] In view of the above situation, in order to solve the defects of the prior art, the purpose of the present invention is to provide a method for simultaneously observing and counting the glandular hairs, non-glandular hairs and stomata of Artemisia argyi. Questions about the distribution of glandular, nonglandular, and stomata

Method used

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  • Piece preparation method for simultaneously observing and counting lower glandular hair, non-glandular hair and stomata of folium artemisiae argyi
  • Piece preparation method for simultaneously observing and counting lower glandular hair, non-glandular hair and stomata of folium artemisiae argyi
  • Piece preparation method for simultaneously observing and counting lower glandular hair, non-glandular hair and stomata of folium artemisiae argyi

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Embodiment 1

[0012] Example 2

[0013] It should be pointed out that the above are only preferred embodiments of the present invention, and are not intended to limit the present invention in any form. Any skilled person who is familiar with the profession, without departing from the scope of the technical solution of the present invention, can use the above disclosed Equivalent embodiments in which the technical content is changed or modified into equivalent changes all fall within the protection scope of the present invention.

[0014] The method for producing slices of the present invention has been repeatedly tried and tested, and the results show that after methanol fixation, not only the shape of the leaf epidermis can be maintained, but also the pigment in the chloroplast can be destroyed, and the interference to the fluorescence color development can be avoided, thereby changing the background of observation. N-hexane can dissolve the waxy layer on the surface of non-glandular hairs...

Embodiment 2

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Abstract

The invention relates to a flaking method for simultaneously observing and counting glandular hairs, non-glandular hairs and stomata of folium artemisiae argyi, which can effectively solve the problem that the distribution conditions of the glandular hairs, the non-glandular hairs and the stomata on the lower surface of the folium artemisiae argyi cannot be simultaneously observed. Soaking and fixing in methanol, rinsing with distilled water, transferring into a sodium hypochlorite solution to be bleached to be colorless, rinsing with distilled water, transferring into a tissue transparent agent to be transparent, taking out the material, dyeing with a sarranine solution, performing water sealing, and observing with an optical microscope until non-glandular hair stems and glandular hair are dyed into red; the method has the advantages of being easy to operate, low in cost, small in workload, high in success rate and clear in observation, the technical requirement for operators is not high, and the method is an innovation in the aspect of the film making method.

Description

technical field [0001] The invention relates to the field of microscopic identification of plants, in particular to a film making method for simultaneously observing and counting the glandular hairs, non-glandular hairs and stomata of Artemisia argyi. Background technique [0002] Ai ( Artemisia argyi ) is Artemisia (Compositae) Artemisia ) plant, Artemisia argyi is the dried leaf of Artemisia argyi, is commonly used Chinese medicine, has the effect of warming meridians to stop bleeding, expelling cold and relieving pain. Artemisia argyi has a wide range of distribution in my country, except for extremely arid and alpine regions, almost all over the country. It is cultivated in many places across the country. The four famous moxa in history include "Qi AI" in Qichun County, Hubei Province, "Northern AI" in Tangyin County, Henan Province, "Qi AI" in Anguo City, Hebei Province, and "Qi AI" in Ningbo, Zhejiang Province. The "Haiai" in Nanyang City, Henan Province, and the "...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30G01N21/84
CPCG01N1/286G01N1/30G01N21/84G01N2001/2873
Inventor 刘孟奇高富郭涛陈随清
Owner HENAN UNIV OF CHINESE MEDICINE
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