Gynecological fluorescent staining solution as well as preparation method and application thereof

Abstract

The invention discloses a gynecological fluorescent staining solution and a preparation method and application thereof.The gynecological fluorescent staining solution comprises acridine orange, sodium chloride, EDTA-Na, disodium hydrogen phosphate and citric acid, the components can be combined with different cell substances for staining, so that fluorescence of different colors is displayed under the action of exciting light, the components are clear after staining, and the staining effect is good. Different components present different colors and are easy to identify and distinguish. The preparation method of the gynecological fluorescent staining solution is simple, and the gynecological fluorescent staining solution can be prepared and used at any time and can also be stored and used after being prepared. According to the use method of the gynecological fluorescent staining solution, two nucleic acids, namely DNA and RNA, are mainly displayed, so that the fluorescent staining solution is obvious in luminescence. The use method is simple, observation and detection can be carried out through a fluorescence microscope after the gynecological fluorescent staining solution is dropwise added, the defects of a traditional saline smear method are overcome, and the detection rate is increased.

Description

A kind of gynecological fluorescent dyeing solution and its preparation method and application technical field The invention belongs to the technical field of medical laboratory supplies, relate to a kind of gynecological fluorescent dyeing solution and preparation method thereof and application thereof. use. Background technique Vaginal secretion analysis is the routine item of gynecological examination, for the diseases such as female reproductive system inflammation, tumor, etc. Diagnosis has important clinical significance and is an important basis for clinical diagnosis of vaginal diseases. [0003] The detection of vaginal secretions is mainly carried out on white blood cells, epithelial cells, clue cells, fungi, bacteria, molds, and trichomoniasis. Observation to determine whether it is bacterial vaginosis, candida vaginosis, trichomoniasis vaginosis or aerobic vaginosis. Tradition The detection methods are wet slide microscopy and Gram staining. However, th...

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Problems solved by technology

However, these two methods have great limitations. The wet film microscope detection method can not accurately observe the bacterial morphology of clue cells with small bacterial morphology, and the effect of observing fungal blastospores is not good. Trichomonad is inactivated or dead. The white blood cells are almost uniform and difficult to distinguish; the staining of the Gram staining method requires multiple steps, takes a long time, and the operation is complicated, and the background interference is serious when the result is interpreted

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