Mineralized material for preventing and treating dentin hypersensitivity and resisting caries as well as preparation method and application of mineralized material
A dentin and sensitive technology, applied in dental preparations, dentistry, pharmaceutical formulations, etc., can solve problems such as demineralization and remineralization of dental hard tissues, easy to produce drug-resistant strains, and affect the sealing effect, etc., to achieve product structure stability , Stable biological activity, good safety effect
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[0028] The preparation method of the mineralized material for preventing and treating dentin sensitivity and anti-caries comprises the following steps:
[0029] Step 1, configure calcium chloride (CaCl 2 ) solution. At room temperature, dissolve 1.11 g of calcium chloride powder in 10 mL of distilled water, vortex evenly, and prepare 1 mol / L CaCl 2 solution.
[0030] Step 2, according to the mass-to-volume ratio of 2g:1L, add powdered IgY to fresh DMEM medium (DMEM solution), vortex evenly, place it in a shaker with parameters of 37°C and 180rpm, and incubate for 12 hours to obtain the first step. a mixture. The first mixed solution is a suspension.
[0031] Step 3, add 20 μL of CaCl per ml of the first mixture 2 The ratio of the solution (1mol / L), the CaCl 2 The solution (1 mol / L) was added to the first mixed solution, and the mixture was continuously placed in a shaker with parameters of 37° C. and 180 rpm for 30 min to obtain a mixture.
[0032] Step 4, quickly divid...
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