Intelligent acquisition method of environment DNA (Deoxyribonucleic Acid)

An intelligent collection and environmental technology, applied in the direction of biological material sampling methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of cumbersome operation process, inability to complete automatic sampling, and no consideration of pollution, etc., to improve sampling efficiency effect

Pending Publication Date: 2022-05-20
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

The intelligent sampling scheme used in other fields does not consider the possible contamination of the sample by microorganisms in the aerosol
[0005] That is to say, for the DNA sampling technology in the water environment, there are technical problems in the prior art that the filtrate is removed by switching the exhaust direction of the vacuum pump, the operation process is cumbersome, and automatic sampling cannot be completed.

Method used

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  • Intelligent acquisition method of environment DNA (Deoxyribonucleic Acid)
  • Intelligent acquisition method of environment DNA (Deoxyribonucleic Acid)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] like figure 1 As shown, the present embodiment 1 provides an intelligent collection method of environmental DNA, which is applied to a sampling device, and the sampling device includes: a membrane filter device 2, a water inlet pipe 3, a water outlet pipe 4, a valve body assembly 6, a first The sensor assembly, the second sensor assembly, the gas-liquid dual-purpose pump 13 and the control assembly 8, the membrane changing filtration device 2 includes a membrane storage area and a sampling area, the membrane storage area is used to place a plurality of spare filter membranes, and the sampling area is placed There are current collection filters, collection methods include:

[0047] The control component 8 controls the gas-liquid dual-purpose pump 13 to perform the first suction filtration action on the water sample, so that the water sample flows through the current collection filter membrane from the water inlet pipe 3 and is discharged after passing through the water o...

Embodiment approach

[0049] In the technical solution of the first embodiment above, for the valve body assembly 6, the valve body assembly 6 is arranged on the water inlet pipeline, the valve body assembly 6 includes a valve chamber, and the valve chamber includes a pair of first valves, second valves and The third valve, one end of a pair of first valves close to each other communicates with the valve cavity, and the end of a pair of first valves far away from each other corresponds to the water inlet direction and the water outlet direction of the water inlet pipeline, and the second valve is connected to the valve through an air filter device 11 cavity, when the air enters the valve cavity, it needs to pass through the air filter device 11 to filter the air through the air filter device 11, and the two ends of the third valve correspond to the valve cavity and a stationary liquid storage device 12, fixed The liquid storage device is provided with a stationary liquid; and before marking and stor...

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Abstract

The invention provides an intelligent environment DNA collection method which comprises the following steps: a control assembly controls a gas-liquid dual-purpose pump to carry out first suction filtration action on a water sample, so that the water sample flows through a current collection filter membrane through a water inlet pipeline and then is discharged through a water outlet pipeline, a pair of sampling equipment is rinsed, and after rinsing, the water sample is discharged through a water outlet pipeline; marking and storing the current collection filter membrane after the first suction filtration action; then, the control assembly is used for controlling the gas-liquid dual-purpose pump to perform N suction filtration actions on the water sample, and each suction filtration action in the N suction filtration actions correspondingly uses one standby filter membrane, so that the water sample flows through the corresponding standby filter membrane from the water inlet pipeline and then is discharged through the water outlet pipeline; n is an integer greater than or equal to 2; and marking and storing the standby filter membrane obtained at each time in the N suction filtration actions, so as to achieve the technical purpose of sampling the water sample through a plurality of filter membranes, thereby achieving the technical effect of improving the sampling efficiency.

Description

technical field [0001] The invention belongs to the technical field of water sample collection, in particular to an intelligent collection method of environmental DNA. Background technique [0002] With the advancement of the new global biodiversity governance strategy and the implementation of the United Nations Convention on Biological Diversity, the importance of biodiversity research has become increasingly prominent. With the development of biodiversity investigation and research, large-scale remote continuous collection and filtration of environmental DNA and microorganisms in water bodies is usually required. Different from ordinary sampling scenarios, in environmental DNA sampling or microbial sampling scenarios, it is necessary to pay special attention to avoid sample contamination, pay attention to control the pressure on the filter membrane, pay attention to timely discharge of filtrate, and timely record environmental parameters, so as to ensure the independence ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/14G01D21/02C12M1/26B01D63/08
CPCG01N1/14G01D21/02C12M1/267B01D63/087G01N2001/1418G01N2001/1093Y02A20/20
Inventor 曾宏辉陆苏祥熊凡甘小妮
Owner INST OF AQUATIC LIFE ACAD SINICA
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