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Mutant allele of ZmAMP1 gene and application thereof

An allele and gene technology, applied in the field of alleles, can solve problems such as dwarfing and lack of breeding application value, and achieve the effects of reducing plant height, improving lodging resistance and yield, and promoting early harvesting.

Active Publication Date: 2022-05-13
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the reported mutant alleles of ZmAMP1 lead to extreme dwarf phenotypes (Lv et al. 2014; Li et al. 2019), which have no breeding application value

Method used

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  • Mutant allele of ZmAMP1 gene and application thereof
  • Mutant allele of ZmAMP1 gene and application thereof
  • Mutant allele of ZmAMP1 gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Example 1 F2:3 population phenotype analysis and QTL mapping

[0084] According to the semi-dwarf and early flowering characteristics of the maize mutant grmm, the F2:3 segregation population was constructed by crossing Si273 with grmm. In the F2:3 population, the traits related to plant height (including plant height, height of ear position and height above ear), traits related to flowering period (including heading stage, powder loosening stage, silking stage), ear traits (ear length and ear diameter) and yield-related traits (including ear row number, kernel number per row, 100-grain weight and yield per plant). For each of the tested traits, there were significant differences between parents (Table 1). The coefficients of variation of these traits were between 3.13% and 25.27%, and the broad-sense heritability was between 0.55 and 0.89, indicating that these traits were mainly controlled by genetic factors. Correlation analysis of the tested traits showed that pla...

Embodiment 2

[0098] Example 2 qPH1 Fine Mapping and Allelism Test

[0099] Using plant height as the target trait for qPH1 fine-mapping, it is located in the interval of 273-277cM ( figure 2 A), the location roughly corresponds to the physical location of 281.8-289.3 Mb (B73 reference genome version 3). A preliminary screening of the exchanged individual plants was performed using markers D2 and D25 (see Table 5 for primer sequences), and the positions of D2 and D25 were 281.2 and 289.9 Mb, respectively. Using the BC2F2 population, the QTL region was narrowed down to the region between G2 and R18 (see Table 5 for primer sequences), which is 227 kb in length according to version 3 of the maize B73 reference genome. Using the BC2F3 population, the QTL region between R14 and G13 was further narrowed down to 16 kb (see Table 5 for primer sequences). Two candidate genes (GRMZM2G010353 and GRMZM2G011385) were found in this region ( figure 2 B).

[0100] Table 5 Primer sequence information ...

Embodiment 3

[0110] Example 3 Mining of ZmAMP1 Variation Sites

[0111] First, qPH1-based near-isogenic lines (NILs) were screened in the BC3F3 generation, and NILs containing grmm fragments are called NILs grmm , containing the Si273 fragment called NIL Si273 , extract the RNA of NILs and parental vegetative growth V6 stage young stem tissue, and compare ZmAMP1 between NILs by transcriptome analysis and real-time quantitative PCR (RT-PCR) (NIL Si273 and NIL grmm ) and the difference between the parents (grmm and Si273).

[0112] qRT-PCR showed that NIL Si273 and NIL grmm There was no significant difference between the parental lines and the parental lines ( Figure 4 A), which shows that the semi-dwarf phenotype of grmm is not related to the expression of ZmAMP1. Therefore, the genomic sequence of ZmAMP1_grmm (after the ATG transcription start codon) was compared with that of ZmAMP1 from Si273, B73, CML247, EP1 and F7 (sequences of B73, CML247, EP1 and F7 are from www.maizegdb.org) ...

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Abstract

The invention relates to the technical field of molecular breeding of crops, in particular to a mutant allele of a ZmAMP1 gene and application of the mutant allele. Compared with the encoding protein of the corn wild type ZmAMP1 gene, the encoding protein of the mutant allele of the ZmAMP1 gene provided by the invention has the mutation that arginine is mutated into cysteine at the 200th site. The mutant allele can lead to the semi-dwarf phenotype of corn, improve the lodging resistance and yield, shorten the flowering period and promote early harvesting, so that the newly cultivated corn hybrid can adapt to high latitude regions with lower accumulated temperature, regions easy to lodging and high planting density, and has important breeding value; the method has an important value for promoting corn yield improvement.

Description

technical field [0001] The invention relates to the technical field of crop molecular breeding, in particular to a mutant allele of ZmAMP1 gene and its application. Background technique [0002] At present, researchers have discovered the green revolution genes SD1 and PHT1 in rice and wheat respectively. These green revolution genes have the advantages of reducing plant height, increasing sowing density, improving lodging resistance and increasing yield. [0003] Due to the advantages of high harvest index, lodging resistance and tolerance to high planting densities, the semi-dwarf varieties of rice and wheat have greatly increased the yield of major crops since the 1960s (Peng et al. 1999; Khush 2001; Sasaki et al. 2002). The promotion of these semi-dwarf varieties promoted the increase of rice and wheat yields, so it is called the green revolution. In rice, the green revolution gene SD1 encodes the GA20 oxidase for GA biosynthesis, and SD1 mutants have reduced GA levels...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/48C12N15/57C12Q1/6895
CPCC12N9/485C12Y304/17011C12Q1/6895C12Q2600/13C12Q2600/156Y02A40/146
Inventor 张红伟王国英王建华王平喜付俊杰
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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