Detection method for blood culture positive reporting

A detection method and blood culture technology, which are applied to measurement devices, preparation of test samples, instruments, etc., can solve the problems of reduced detection sensitivity, high cost of blood culture bottles, high background, shortened reporting time, and simple method. Quick and easy to implement

Pending Publication Date: 2022-04-29
厦门元谱生物科技有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Therefore there are following disadvantages: 1) low sensitivity: at first, because carbon dioxide exists with a gaseous state in a part of the culture bottle, and another part is dissolved in the culture solution, so the above-mentioned detection method can only partially sample, which will inevitably reduce the detection sensitivity; secondly, due to The air itself contains more than 0.03% carbon dioxide, so the background is high, which greatly affects the sensitivity; 2) The scope of application is limited: the above detection method can only detect those microorganisms that produce carbon dioxide in the production and metabolism, and it is not suitable for microorganisms that do not produce carbon dioxide. Unable to detect; 3) High cost: In order to detect changes in carbon dioxide, a microbial indicator of carbon dioxide needs to be added to the blood culture bottle with a special process, resulting in high cost of the blood culture bottle

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  • Detection method for blood culture positive reporting
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  • Detection method for blood culture positive reporting

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preparation example Construction

[0024] In the present invention, the matrix liquid preferably includes the following raw materials: matrix, organic solvent, deionized ultrapure water and trifluoroacetic acid. The matrix preferably includes an organic matrix or an inorganic matrix. The organic matrix preferably includes cinnamic acid or 2,5-dihydroxybenzoic acid. The present invention is not particularly limited to the specific type of the inorganic matrix, and the conventional inorganic matrix types in the art are used. can be. The present invention has no special limitation on the specific source of each raw material in the matrix liquid, and conventional commercially available products in this field can be used. In the present invention, the volume ratio of the organic solvent to deionized ultrapure water is preferably 10:0-1:9, more preferably 7:3, the organic solvent preferably includes acetonitrile, and the deionized ultrapure water The specification of the water is preferably resistivity>18MΩ.cm, and ...

Embodiment 1

[0029] Take the sample (containing liquid medium and blood) in the 500uL blood culture bottle, pour it into the vacuum separation gel blood collection tube, after the blood is completely coagulated, centrifuge at 3000 rpm for 10 minutes to obtain the serum. Extract 100uL of the above serum, use a 3kDa ultrafiltration membrane to remove blood cells and large proteins, and take the supernatant for sample injection.

[0030]Weigh 10 mg of cinnamic acid and dissolve in 100 mL of a mixture of acetonitrile and deionized ultrapure water (V:V=70:30), deionized ultrapure water (resistivity>18MΩ.cm), and finally add 0.11% trifluoro Acetic acid to prepare matrix solution. Mix 50uL of the above supernatant with 50uL of matrix solution to obtain the sample to be tested and wait for sample application.

[0031] Use a pipette to spot 1uL of the above-mentioned sample to be tested on the target plate, 10 spots for each sample. After the drying and crystallization is completed, it is sent to...

Embodiment 2

[0033] Take the sample (containing liquid medium and blood) in the 1000uL blood culture bottle, inject it into the vacuum separation gel blood collection tube, after the blood is completely coagulated, centrifuge at 3000 rpm for 10 minutes to obtain the serum. Take 100uL of the above serum, add methanol organic solvent according to the volume ratio of 1:10, centrifuge at 12000 rpm for 10 minutes, take the supernatant and wait for sample injection.

[0034] Weigh 10mg of 2,5-dihydroxybenzoic acid and dissolve in 100mL of a mixture of acetonitrile and deionized ultrapure water (V:V=1:9), deionized ultrapure water (resistivity>18MΩ.cm), and finally Add 5% trifluoroacetic acid to prepare matrix solution. Mix 50uL of the above supernatant with 50uL of matrix solution to obtain the sample to be tested and wait for sample application.

[0035] The electrospray ion source is used, 1uL is injected each time, and the mass range is set to 10-1000Da. The positive ion mode is also used to...

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Abstract

The invention provides a detection method for blood culture positive reporting, and belongs to the technical field of blood sample detection. The blood culture positive reporting detection method comprises the following steps: mixing a blood culture bottle sample and a matrix liquid, monitoring small molecule components with the molecular weight of 10-1000Da in a blood culture bottle by adopting a mass spectrometry, and comparing the change of the small molecule components by applying an AI clustering analysis algorithm to realize the positive reporting detection of the blood culture bottle. According to the present invention, the advantages of high sensitivity of the mass spectrum and simultaneous detection of a variety of molecules are utilized, and the AI algorithm is combined, such that the blood culture positive reporting time can be substantially shortened so as to reduce the incidence rate and the mortality of the infection.

Description

technical field [0001] The invention belongs to the technical field of blood sample detection, and in particular relates to a detection method for positive results of blood culture. Background technique [0002] The detection of positive blood culture is a microbiological examination method used to test the presence or absence of bacteria in blood samples. It inoculates blood into a culture bottle, uses the nutrient solution in the culture bottle to cultivate the microorganisms infected by the blood, and then monitors the production and reproduction of the microorganisms. If there are microorganisms detected, a positive alarm is issued. Therefore, the positive reporting rate and positive reporting speed are the two most important indicators for blood culture positive testing. [0003] Existing blood culture positive detection methods are mainly as follows: 1) VITAL automatic blood culture (CN201280067584.3) produced by French bioMerieux company, its detection principle is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N27/64G01N27/68
CPCG01N1/28G01N27/64G01N27/68
Inventor 何浩睿
Owner 厦门元谱生物科技有限公司
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