Application of circular RNA-circEXOC6B in inhibiting metastasis of prostate cancer
A technology for prostate cancer and prostate, which is applied in the field of biomedicine and can solve problems such as the inhibition of prostate cancer by circular RNA-circEXOC6B.
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[0034] 1. Method
[0035]1. Construction of CircEXOC6B (hsa_circ_0009043) overexpression plasmid vector
[0036] 1) Overexpression plasmid construction
[0037] The overexpression plasmid vector was constructed by Novopro Company through gene synthesis. Plasmid structure such as Figure 4 As shown, the expression sequence of back circular frame+circEXOC6B+front circular frame is shown in SEQ ID NO: 4, wherein the expression sequence of circEXOC6B is the 664-1053 bp of SEQ ID NO: 4.
[0038] 2) Cell transfection
[0039] Cell transfection was divided into two groups, namely circEXOC6B overexpression plasmid group and empty plasmid group (PLVX-PURO) or NC group. 48h after transfection, DU145 and PC-3 cells were collected.
[0040] 3) Design circEXOC6B primers and verify their specificity
[0041] Firstly, the total RNA of collected cells is extracted, and then reverse transcribed into cDNA. Design specific primers for cirEXOC6B. The sequence is as follows: Forward: CCTCA...
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