Primer combination, kit, model, construction method and detection method for detecting BRAF gene mutation

Abstract

The invention relates to the field of molecular biological detection, in particular to a primer combination, a kit, a model, a construction method and a detection method for detecting BRAF gene mutation. Comprising the following steps: extracting a cfDNA sample, configuring a reaction system for detecting BRAF gene mutation based on the extracted cfDNA sample, correspondingly performing PCR amplification, performing linker addition and PCR enrichment on an amplification product to prepare a sequencing library, performing quantification and quality control on the sequencing library, performing high-throughput sequencing by adopting a gene sequencer, and analyzing sequencing data by adopting biological information analysis software. According to the detection method, a free nucleic acid sample containing 0.09% of BRAF gene related site mutation can be detected for the 12th exon of the BRAF gene, and a free nucleic acid sample containing 0.05% of BRAF gene related site mutation can be detected for the 15th exon of the BRAF gene; the biological clinical detection level of BRAF gene related sites can be improved, and a foundation is laid for popularization and application.

Description

technical field [0001] The invention relates to the field of molecular biology detection, in particular to a primer combination, kit, model, construction method and detection method for detecting BRAF gene mutation. Background technique [0002] The clinical features and prognostic factors of Langerhans cell histiocytosis (LCH) in adults are unclear. However, recurrent somatic mutations of the BRAF V600E allele have been found in LCH, and most of the genome analysis comes from children. In newly diagnosed LCH tissue samples, the first-generation package plus the second-generation package gene exon mutation detection, 63.2 % of the children had BRAF gene mutations, and the mutation sites were mainly concentrated in the 12th and 15th exons. The p.V600E type accounted for 50.6%; the positive rate of non-p.V600E mutations was 12.6%, including: p.N486_T491delinsK, p.V600D(c.1799_1800delinsAT), p.N486_P490del, p.L485_P490delinsF, and three sporadic positive mutations (p.V600D(c....

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Problems solved by technology

[0004] In view of the above-mentioned deficiencies in the prior art, the problem to be solved in the present invention is: how to provide a primer combination, kit, model, construction method and detection method for detecting BRAF gene mutations, so as to solve the problem of BRAF gene mutation in the prior art. Inconvenient detection of related site mutations

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