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Multifunctional nanoparticle based on metal organic framework as well as preparation method and application of multifunctional nanoparticle

A metal-organic framework and multi-functional technology, applied in medical preparations with non-active ingredients, medical preparations containing active ingredients, nanotechnology, etc., can solve the problems of easy activation of cancer cells and unsatisfactory curative effect, and achieve optimal treatment The effect of the program

Active Publication Date: 2022-04-15
THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention intends to provide a metal-organic framework-based multifunctional nanoparticle to solve the technical problem that cancer cells are prone to initiate hypoxia-related self-protection pathways during photothermal or photodynamic therapy, resulting in unsatisfactory curative effects

Method used

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  • Multifunctional nanoparticle based on metal organic framework as well as preparation method and application of multifunctional nanoparticle
  • Multifunctional nanoparticle based on metal organic framework as well as preparation method and application of multifunctional nanoparticle
  • Multifunctional nanoparticle based on metal organic framework as well as preparation method and application of multifunctional nanoparticle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Preparation of multifunctional nanoparticles based on metal organic framework

[0044] 100mg 4-carboxyphenylporphyrin (TCPP), 300mg zirconium oxychloride octahydrate (ZrOCl 2 ·8H 2 O), 2.8g benzoic acid (benzoic acid), dispersed in 100ml N,N'-dimethylformamide (DMF), stirred at 90°C (optional temperature range is 85-90°C) for 5h, centrifuged at 12000rpm for 10min for purification , take the solid phase after centrifugation to obtain the metal organic framework. The aqueous solution of AQ4N and metal-organic framework was stirred at room temperature in the dark for 24 h, and AP NPs (PCN-224@AQ4N) were obtained after centrifugation. Wherein, in the above aqueous solution, the mass ratio of AQ4N to the metal organic framework is 1:1 (the optional mass ratio is 0.5-1.5:0.5-1.5), and the concentration of the metal organic framework in the aqueous solution is 1 mg / mL.

[0045] Disperse 10mg of AP NPs into absolute ethanol-water solution (15ml, 3:4, v:v), ultraso...

Embodiment 2

[0046] Embodiment 2: Preparation of non-drug-loaded nanoparticles (PP NPs)

[0047] 100mg 4-carboxyphenylporphyrin (TCPP), 300mg zirconium oxychloride octahydrate (ZrOCl 2 ·8H 2 O), 2.8g benzoic acid (benzoic acid), dispersed in 100ml N,N'-dimethylformamide (DMF), stirred at 90°C for 5h, centrifuged at 12000rpm for 10min for purification, and the solid phase after centrifugation was taken to obtain a metal-organic framework .

[0048] Take 10mg of metal organic framework and disperse it into absolute ethanol-water solution (15ml, 3:4, v:v), ultrasonically disperse for 4min (65%, 40KHz), and then add 15mg of dopamine hydrochloride, wherein, the mixture of metal organic framework and dopamine hydrochloride The mass ratio is maintained at a ratio of 1:0.8-2.0. Stir magnetically for 8 minutes, then add 20 ml of Tris-Hcl with a pH value of 8.5-8.8 to the mixture to adjust the pH of the mixture to alkaline 8.5, and stir (600 rpm) in the dark for 24 hours. The obtained product wa...

experiment example 1

[0049] Experimental example 1: Characterization of multifunctional nanoparticles

[0050] According to the conventional methods of the prior art, observe the surface morphology of the nanoparticles of Example 1, see the transmission electron microscope image figure 1 , SEM image see figure 2 . Measure the particle diameter and Zeta potential of the nanoparticle of embodiment 1, particle diameter and potential distribution figure see respectively image 3 with Figure 4 . The particle size of the nanoparticles prepared in Example 1 is 91±5.5nm, and the surface potential is -7.8±0.8mV. Encapsulation efficiency and drug loading were measured with reference to the following methods: encapsulation efficiency (%w / w)=(drug / medicine addition in nanocomposite)×100%; drug loading (%w / w)=(nanocomposite drug / nanocomplex total mass) × 100%. The encapsulation efficiency of AQ4N was 49.16%, and the drug loading was 19.98%.

[0051] PCN-224 and AQ4N were incubated at different mass ra...

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Abstract

The invention relates to the technical field of nano-drug presentation systems and contrast agents, in particular to multifunctional nanoparticles based on a metal organic framework as well as a preparation method and application of the multifunctional nanoparticles. A multifunctional nanoparticle based on a metal organic framework comprises the metal organic framework, and the metal organic framework is loaded with a medicine AQ4N activated under an anoxic condition and is modified with polydopamine. The nanoparticle can solve the technical problem of unsatisfactory curative effect caused by oxygen deficit of tumor tissues in the process of pure photo-thermal or photodynamic therapy, is a multi-stimulus inertial response nano system, and can overcome the inherent defects of multiple biological obstacles and a single-stimulus response system; the method has great application value in noninvasive diagnosis and treatment of tumors.

Description

technical field [0001] The invention relates to the technical field of nano drug delivery system and contrast agent, in particular to a multifunctional nano particle based on metal organic framework and its preparation method and application. Background technique [0002] Light-based therapeutic approaches such as photothermal therapy (PTT) and photodynamic therapy (PDT) have gained popularity in cancer treatment due to their non-invasiveness, high spatiotemporal precision, controllability, negligible drug resistance, and localized treatment. received widespread attention. Although light-based therapeutics have received extensive attention in cancer treatment, how to improve the specificity and effectiveness of this therapeutic approach remains a great challenge. The abnormal physical and chemical tumor microenvironment, hypoxia, and acidic pH value caused by tumor autologous disorders and irregular blood vessel distribution induce or participate in the generation of drug r...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K47/59A61K41/00A61K31/14G01N21/17A61P35/00B82Y5/00B82Y40/00
CPCY02A50/30
Inventor 张伟冉海涛
Owner THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV
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