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Chloroplast genome of Sancinnamomum huanggong pepper and application thereof

A technology for chloroplast genome and Sanshanghuanggongjiao, applied in the field of genes, can solve the problems such as no research and report on the chloroplast genome of Sanshanghuanggongjiao, miscellaneous varieties, inability to complete systematic classification, etc., and achieves good specificity and detection cost. Reduce, detect the effect of accurate

Pending Publication Date: 2022-04-12
衡阳市蔬菜研究所
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  • Description
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AI Technical Summary

Problems solved by technology

However, there are various, chaotic, and many varieties of three camphor yellow tribute peppers. Among different types of peppers, it is impossible to complete the systematic classification only by morphological characteristics.
At present, there is no research or report on the chloroplast genome of Campylos camphorata

Method used

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  • Chloroplast genome of Sancinnamomum huanggong pepper and application thereof
  • Chloroplast genome of Sancinnamomum huanggong pepper and application thereof
  • Chloroplast genome of Sancinnamomum huanggong pepper and application thereof

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Embodiment Construction

[0037] The present invention will be described in detail through specific examples below. These embodiments are provided for a more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.

[0038] "Includes" or "comprises" mentioned throughout the specification and claims is an open term, so it should be interpreted as "including but not limited to". The subsequent description in the specification is a preferred implementation mode for implementing the present invention, but the description is for the purpose of the general principles of the specification, and is not intended to limit the scope of the present invention. The scope of protection of the present invention should be defined by the appended claims.

[0039] Unless otherwise specified, various reagents and materials used in the present invention can be purchased from the market.

[0040] 1. Extraction of total DNA from the sample

[0041] Th...

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Abstract

The chloroplast genome of the Sanshan yellow pepper is disclosed for the first time and is applied to the phylogenetic research of the Sanshan yellow pepper, and the phylogenetic status of the Sanshan yellow pepper in capsicum is analyzed. According to the method, molecular systematics and phylogenomics methods are utilized, the phylogenetic relationship of capsicum species is established through a Sanshan yellow pepper chloroplast genome, and on the basis, the Sanshan yellow pepper chloroplast whole genome is used as a super DNA bar code of capsicum and is used for identifying the variety of Sanshan yellow pepper. The method provides theoretical basis and technical support for protection and species identification of germplasm resources of capsicum, especially Sanshan Huanggong pepper, and has important significance for breeding of Sanshan Huanggong pepper and genetic diversity evaluation, protection and utilization of Sanshan Huanggong pepper.

Description

technical field [0001] The invention belongs to the field of gene technology, and in particular relates to the chloroplast genome of camphor yellow pepper and its application. Background technique [0002] With the development of society, human beings have increasingly urgent needs for the exploration of biodiversity, the rational use of biological resources and the rapid identification of species, and DNA barcoding technology has emerged as the times require. DNA barcode refers to a standard DNA sequence used for species identification, which is similar to the DNA barcode used to distinguish commodities and is used to distinguish species. For example, more than 200 species of Lepidoptera insects can be identified by using a fragment of about 680 bp in length of the mitochondrial cytochrome oxidase subunit 1 (cytochrome oxidase I, COI) gene. Each species has a DNA sequence that is different from other species. By comparing the DNA sequence of the sample with the reference s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12Q1/6895C12Q1/6869G16B30/10G16B30/20G16B25/20G16B50/30
Inventor 唐可兰刘志华余席茂马璐李健生文明英刘阳华唐锷谭美丽肖昌华陈琳何振华刘剑眉谭建军李建忠文立丰
Owner 衡阳市蔬菜研究所
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